Quantifying the Contribution of Entire Free-Living Nematode Communities to Carbon Mineralization under Contrasting C and N Availability

• Published in: PloS one 2015-09, Vol.10 (9), p.e0136244-e0136244
• Main Authors: Gebremikael, Mesfin Tsegaye, Steel, Hanne, Bert, Wim, Maenhout, Peter, Sleutel, Steven, De Neve, Stefaan
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Biochemistry; Biology; Biomass; Carbon; Carbon - metabolism; Cellulases - metabolism; Clover; Communities; Decomposition; Enzymatic activity; Experiments; Fatty Acids - metabolism; Food chains; Food sources; Gas Chromatography-Mass Spectrometry; Gram-Negative Bacteria - metabolism; Gram-Positive Bacteria - metabolism; Incubation; Metabolism; Microbiota (Symbiotic organisms); Microflora; Microorganisms; Mineralization; Nematoda - enzymology; Nematoda - metabolism; Nematodes; Nitrogen; Nitrogen - metabolism; Organic matter; Oxidoreductases - metabolism; Poaceae - growth & development; Poaceae - metabolism; Prey; Protozoa; Respiration; Roundworms; Soil - chemistry; Soil carbon; Soil Microbiology; Soil microorganisms; Soils
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0136244

To understand the roles of nematodes in organic matter (OM) decomposition, experimental setups should include the entire nematode community, the native soil microflora, and their food sources. Yet, published studies are often based on either simplified experimental setups, using only a few selected species of nematode and their respective prey, despite the multitude of species present in natural soil, or on indirect estimation of the mineralization process using O2 consumption and the fresh weight of nematodes. We set up a six-month incubation experiment to quantify the contribution of the entire free living nematode community to carbon (C) mineralization under realistic conditions. The following treatments were compared with and without grass-clover amendment: defaunated soil reinoculated with the entire free living nematode communities (+Nem) and defaunated soil that was not reinoculated (-Nem). We also included untreated fresh soil as a control (CTR). Nematode abundances and diversity in +Nem was comparable to the CTR showing the success of the reinoculation. No significant differences in C mineralization were found between +Nem and -Nem treatments of the amended and unamended samples at the end of incubation. Other related parameters such as microbial biomass C and enzymatic activities did not show significant differences between +Nem and -Nem treatments in both amended and unamended samples. These findings show that the collective contribution of the entire nematode community to C mineralization is small. Previous reports in literature based on simplified experimental setups and indirect estimations are contrasting with the findings of the current study and further investigations are needed to elucidate the extent and the mechanisms of nematode involvement in C mineralization.