Biodiversity, Anti-Trypanosomal Activity Screening, and Metabolomic Profiling of Actinomycetes Isolated from Mediterranean Sponges

Marine sponge-associated actinomycetes are considered as promising sources for the discovery of novel biologically active compounds. In the present study, a total of 64 actinomycetes were isolated from 12 different marine sponge species that had been collected offshore the islands of Milos and Crete...

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Bibliographic Details
Published in:PloS one 2015-09, Vol.10 (9), p.e0138528-e0138528
Main Authors: Cheng, Cheng, MacIntyre, Lynsey, Abdelmohsen, Usama Ramadan, Horn, Hannes, Polymenakou, Paraskevi N, Edrada-Ebel, RuAngelie, Hentschel, Ute
Format: Article
Language:English
Subjects:
Acids
Actinobacteria - chemistry
Actinobacteria - classification
Actinobacteria - metabolism
Actinomycetes
Actinomycetospora
Animals
Antiprotozoal Agents - isolation & purification
Antiprotozoal Agents - pharmacology
Bacteria
Bioactive compounds
Biodiversity
Biological activity
Biological Products - isolation & purification
Biological Products - pharmacology
Collection
Discriminant analysis
Drug Discovery
Feasibility studies
Gene sequencing
Geodermatophilus
Hypotheses
Medical screening
Mediterranean Sea
Metabolism
Metabolites
Metabolome
Metabolomics
Microlunatus
Micromonospora
Molecular Sequence Data
Multivariate analysis
NMR
Nuclear magnetic resonance
Parasitic Sensitivity Tests
Pharmacy
Phylogeny
Porifera - microbiology
Principal components analysis
R&D
Research & development
Rhodococcus
rRNA 16S
Sponges
Strains (organisms)
Streptomyces
Taxonomy
Trypanosoma brucei
Trypanosoma brucei brucei - drug effects
Trypanosoma brucei brucei - growth & development
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Summary:Marine sponge-associated actinomycetes are considered as promising sources for the discovery of novel biologically active compounds. In the present study, a total of 64 actinomycetes were isolated from 12 different marine sponge species that had been collected offshore the islands of Milos and Crete, Greece, eastern Mediterranean. The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. Four putatively novel species belonging to genera Geodermatophilus, Microlunatus, Rhodococcus and Actinomycetospora were identified based on a 16S rRNA gene sequence similarity of < 98.5% to currently described strains. Eight actinomycete isolates showed bioactivities against Trypanosma brucei brucei TC221 with half maximal inhibitory concentration (IC50) values
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0138528