Circulating microRNA-200 Family as Diagnostic Marker in Hepatocellular Carcinoma

In this clinical study, we aimed to evaluate the role of circulating microRNA-200 family as a non-invasive tool to identify patients with cirrhosis-associated hepatocellular carcinoma (HCC). Prognosis of HCC remains poor with increasing incidence worldwide, mainly related to liver cirrhosis. So far,...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-10, Vol.10 (10), p.e0140066-e0140066
Main Authors: Dhayat, Sameer A, Hüsing, Anna, Senninger, Norbert, Schmidt, Hartmut H, Haier, Jörg, Wolters, Heiner, Kabar, Iyad
Format: Article
Language:English
Subjects:
Adult
Aged
Analysis
Biological markers
Biomarkers
Biomarkers, Tumor - blood
Blood
Blood circulation
Carcinoma, Hepatocellular - blood
Carcinoma, Hepatocellular - diagnosis
Case-Control Studies
Cell adhesion & migration
Cirrhosis
Deregulation
Diagnosis
Diagnosis, Differential
Diagnostic systems
Family medical history
Female
Gene expression
Genetic aspects
Hepatitis
Hepatocellular carcinoma
Hospitals
Humans
Liver
Liver cancer
Liver cirrhosis
Liver Cirrhosis - blood
Liver Cirrhosis - diagnosis
Liver diseases
Liver Neoplasms - blood
Liver Neoplasms - diagnosis
Male
Medical diagnosis
Medicine
MicroRNA
MicroRNAs
MicroRNAs - blood
Middle Aged
miRNA
Patients
Plasma
Ribonucleic acid
RNA
ROC Curve
Statistical analysis
Surgery
Systematic review
Target detection
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this clinical study, we aimed to evaluate the role of circulating microRNA-200 family as a non-invasive tool to identify patients with cirrhosis-associated hepatocellular carcinoma (HCC). Prognosis of HCC remains poor with increasing incidence worldwide, mainly related to liver cirrhosis. So far, no reliable molecular targets exist for early detection of HCC at surgically manageable stages. Recently, we identified members of the microRNA-200 family as potential diagnostic markers of cirrhosis-associated HCC in patient tissue samples. Their value as circulating biomarkers for HCC remained undefined. Blood samples and clinicopathological data of consecutive patients with liver diseases were collected prospectively. Expression of the microRNA-200 family was investigated by qRT-PCR in blood serum samples of 22 HCC patients with and without cirrhosis. Serum samples of patients with non-cancerous chronic liver cirrhosis (n = 22) and of healthy volunteers (n = 15) served as controls. MicroRNA-141 and microRNA-200a were significantly downregulated in blood serum of patients with HCC compared to liver cirrhosis (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0140066