Chitosan Treatment Delays the Induction of Senescence in Human Foreskin Fibroblast Strains

Fibroblasts have been extensively used as a model to study cellular senescence. The purpose of this study was to investigate whether the human foreskin fibroblast aging process could be regulated by using the biomaterial chitosan. Fibroblasts cultured on commercial tissue culture polystyrene (TCPS)...

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Published in:PloS one 2015-10, Vol.10 (10), p.e0140747-e0140747
Main Authors: Tsai, Ching-Wen, Kao, Yu-Ting, Chiang, I-Ni, Wang, Jyh-Horng, Young, Tai-Horng
Format: Article
Language:English
Subjects:
Aging
Apoptosis
Biomaterials
Biomedical engineering
Biomedical materials
Bone surgery
Cancer
CD3 antigen
Cell aging
Cell culture
Cell Cycle - drug effects
Cell Movement - drug effects
Cell size
Cell Survival - drug effects
Cells, Cultured
Cellular Senescence - drug effects
Cellular Senescence - genetics
Chitin
Chitosan
Chitosan - pharmacology
Engineering schools
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - metabolism
Foreskin - cytology
Foreskin - metabolism
Galactosidase
Gene Expression
Hospitals
Humans
Male
Markers
Medicine
p53 Protein
Physiological aspects
Polystyrene
Polystyrene resins
Prevention
Proteins
Retina
Retinoblastoma
Senescence
Spheroids
Tissue culture
Tissue engineering
Tumorigenesis
β-Galactosidase
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cited_by cdi_FETCH-LOGICAL-c692t-b7e8d0207a6882860a55adbc3b3bdc7c07464123cf48a1f75fea662f2619aaca3
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creator Tsai, Ching-Wen
Kao, Yu-Ting
Chiang, I-Ni
Wang, Jyh-Horng
Young, Tai-Horng
description Fibroblasts have been extensively used as a model to study cellular senescence. The purpose of this study was to investigate whether the human foreskin fibroblast aging process could be regulated by using the biomaterial chitosan. Fibroblasts cultured on commercial tissue culture polystyrene (TCPS) entered senescence after 55-60 population doublings (PDs), and were accompanied by larger cell shape, higher senescence-associated β-galactosidase (SA β-gal) activity, lower proliferation capacity, and upregulation of senescence-associated molecular markers p21, p53, retinoblastoma (pRB), and p16. Before senescence was reached, PD48 cells were collected from TCPS and seeded on chitosan for three days (PD48-Cd3) to form multicellular spheroids. The protein expression of senescence-associated secretory phenotypes (SASPs) and senescence-associated molecular markers of these cells in PD48-Cd3 spheroids were downregulated significantly. Following chitosan treatment, fibroblasts reseeded on TCPS showed lower SA β-gal activity, increased cellular motility, and a higher proliferation ability of 70-75 PDs. These phenotypic changes were not accompanied by colonies forming in soft agar and a continuous decrease in the senescence-associated proteins p53 and pRB which act as a barrier to tumorigenesis. These results demonstrate that chitosan treatment could delay the induction of senescence which may be useful and safe for future tissue engineering applications.
doi_str_mv 10.1371/journal.pone.0140747
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subjects Aging
Apoptosis
Biomaterials
Biomedical engineering
Biomedical materials
Bone surgery
Cancer
CD3 antigen
Cell aging
Cell culture
Cell Cycle - drug effects
Cell Movement - drug effects
Cell size
Cell Survival - drug effects
Cells, Cultured
Cellular Senescence - drug effects
Cellular Senescence - genetics
Chitin
Chitosan
Chitosan - pharmacology
Engineering schools
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - metabolism
Foreskin - cytology
Foreskin - metabolism
Galactosidase
Gene Expression
Hospitals
Humans
Male
Markers
Medicine
p53 Protein
Physiological aspects
Polystyrene
Polystyrene resins
Prevention
Proteins
Retina
Retinoblastoma
Senescence
Spheroids
Tissue culture
Tissue engineering
Tumorigenesis
β-Galactosidase
title Chitosan Treatment Delays the Induction of Senescence in Human Foreskin Fibroblast Strains
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