Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco

Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure....

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-10, Vol.10 (10), p.e0140591-e0140591
Main Authors: Xiang, Yang, Lu, Yun Hai, Song, Min, Wang, Yun, Xu, Wenqi, Wu, Lintao, Wang, Hancheng, Ma, Zhengqiang
Format: Article
Language:English
Subjects:
Abiotic stress
Analysis
Arabidopsis
Arabidopsis thaliana
Bioinformatics
Calcium
Calreticulin
Calreticulin - genetics
Calreticulin - metabolism
Catalase
Catalase - metabolism
Cell adhesion & migration
Chinese cabbage
Cloning, Molecular
Gene expression
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genes
Genetic aspects
Genetic engineering
Genomics
Germplasm
Growth conditions
Homeostasis
Homology
Kinases
Laboratories
Nicotiana - genetics
Nicotiana - physiology
Organ Specificity
Oryza
Peroxidase
Peroxidase - metabolism
Physiological aspects
Physiology
Plant Proteins - genetics
Plant Proteins - metabolism
Plant sciences
Plant tissues
Plants (botany)
Plants, Genetically Modified - genetics
Plants, Genetically Modified - physiology
Proteins
Salinity
Salinity tolerance
Salts
Stress
Stress, Physiological
Stresses
Superoxide dismutase
Superoxide Dismutase - metabolism
Tobacco
Transgenic plants
Triticum - genetics
Triticum - metabolism
Triticum aestivum
Wheat
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c692t-3642971bc3b8d06ae7994a4c787f25082e76deebfb354c684069f1a2059f5403
cites cdi_FETCH-LOGICAL-c692t-3642971bc3b8d06ae7994a4c787f25082e76deebfb354c684069f1a2059f5403
container_end_page e0140591
container_issue 10
container_start_page e0140591
container_title PloS one
container_volume 10
creator Xiang, Yang
Lu, Yun Hai
Song, Min
Wang, Yun
Xu, Wenqi
Wu, Lintao
Wang, Hancheng
Ma, Zhengqiang
description Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure. In this study, three TaCRT genes were identified in wheat and named TaCRT1, TaCRT2 and TaCRT3-1 based on their sequence characteristics and their high homology to other known CRT genes. Quantitative real-time PCR expression data revealed that these three genes exhibit different expression patterns in different tissues and are strongly induced under salt stress in wheat. The calcium-binding properties of the purified recombinant TaCRT1 protein were determined using a PIPES/Arsenazo III analysis. TaCRT1 gene overexpression in Nicotiana tabacum decreased salt stress damage in transgenic tobacco plants. Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions. Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.
doi_str_mv 10.1371/journal.pone.0140591
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1722473293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A431677021</galeid><doaj_id>oai_doaj_org_article_3caa50497b004d009d73af5c1bb3e4b8</doaj_id><sourcerecordid>A431677021</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-3642971bc3b8d06ae7994a4c787f25082e76deebfb354c684069f1a2059f5403</originalsourceid><addsrcrecordid>eNqNk99r2zAQx83YWLts_8HYDIPRPiSTLFmyXgYl7EegEGjNXoUsnx0VxcokO7T__ZTFLfHow9CDxOlz39Od7pLkPUYLTDj-cucG3ym72LkOFghTlAv8IjnHgmRzliHy8uR8lrwJ4Q6hnBSMvU7OMkaZKHJxnrTrPXi433kIwbgudU2q0tKb3uhhmyoIvdnHw1JZDwebNV3aQgfpRamWNyW-TFfbnXd7COmtipemf0hLZ8GrTkMa4dJVSmv3NnnVKBvg3bjPkvL7t3L5c369_rFaXl3PNRNZPyeMZoLjSpOqqBFTwIWgimpe8CbLUZEBZzVA1VQkp5oVFDHRYJXF5JucIjJLPh5ld9YFOZYoSMyzjHKSCRKJ1ZGonbqTO2-2yj9Ip4z8a3C-lcrHTC1IopXKERW8QojWCImaE9XkGlcVAVoVUevrGG2otlBr6Hqv7ER0etOZjWzdXlKGOEU4ClyMAt79HmKx5dYEDdaqDtxwfLcgCMePmyWf_kGfz26kWhUTMF3jYlx9EJVXlGDGOcoOYRfPUHHVsDU69lNjon3icDlxiEwP932rhhDk6vbm_9n1ryn7-YTdgLL9Jjg79LEVwxSkR1B7F4KH5qnIGMnDODxWQx7GQY7jEN0-nH7Qk9Nj_5M_OLID8w</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1722473293</pqid></control><display><type>article</type><title>Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Xiang, Yang ; Lu, Yun Hai ; Song, Min ; Wang, Yun ; Xu, Wenqi ; Wu, Lintao ; Wang, Hancheng ; Ma, Zhengqiang</creator><contributor>Zhang, Jin-Song</contributor><creatorcontrib>Xiang, Yang ; Lu, Yun Hai ; Song, Min ; Wang, Yun ; Xu, Wenqi ; Wu, Lintao ; Wang, Hancheng ; Ma, Zhengqiang ; Zhang, Jin-Song</creatorcontrib><description>Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure. In this study, three TaCRT genes were identified in wheat and named TaCRT1, TaCRT2 and TaCRT3-1 based on their sequence characteristics and their high homology to other known CRT genes. Quantitative real-time PCR expression data revealed that these three genes exhibit different expression patterns in different tissues and are strongly induced under salt stress in wheat. The calcium-binding properties of the purified recombinant TaCRT1 protein were determined using a PIPES/Arsenazo III analysis. TaCRT1 gene overexpression in Nicotiana tabacum decreased salt stress damage in transgenic tobacco plants. Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions. Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0140591</identifier><identifier>PMID: 26469859</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abiotic stress ; Analysis ; Arabidopsis ; Arabidopsis thaliana ; Bioinformatics ; Calcium ; Calreticulin ; Calreticulin - genetics ; Calreticulin - metabolism ; Catalase ; Catalase - metabolism ; Cell adhesion &amp; migration ; Chinese cabbage ; Cloning, Molecular ; Gene expression ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Genes ; Genetic aspects ; Genetic engineering ; Genomics ; Germplasm ; Growth conditions ; Homeostasis ; Homology ; Kinases ; Laboratories ; Nicotiana - genetics ; Nicotiana - physiology ; Organ Specificity ; Oryza ; Peroxidase ; Peroxidase - metabolism ; Physiological aspects ; Physiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant sciences ; Plant tissues ; Plants (botany) ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - physiology ; Proteins ; Salinity ; Salinity tolerance ; Salts ; Stress ; Stress, Physiological ; Stresses ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Tobacco ; Transgenic plants ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum ; Wheat</subject><ispartof>PloS one, 2015-10, Vol.10 (10), p.e0140591-e0140591</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Xiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Xiang et al 2015 Xiang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3642971bc3b8d06ae7994a4c787f25082e76deebfb354c684069f1a2059f5403</citedby><cites>FETCH-LOGICAL-c692t-3642971bc3b8d06ae7994a4c787f25082e76deebfb354c684069f1a2059f5403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1722473293/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1722473293?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26469859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zhang, Jin-Song</contributor><creatorcontrib>Xiang, Yang</creatorcontrib><creatorcontrib>Lu, Yun Hai</creatorcontrib><creatorcontrib>Song, Min</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><creatorcontrib>Xu, Wenqi</creatorcontrib><creatorcontrib>Wu, Lintao</creatorcontrib><creatorcontrib>Wang, Hancheng</creatorcontrib><creatorcontrib>Ma, Zhengqiang</creatorcontrib><title>Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure. In this study, three TaCRT genes were identified in wheat and named TaCRT1, TaCRT2 and TaCRT3-1 based on their sequence characteristics and their high homology to other known CRT genes. Quantitative real-time PCR expression data revealed that these three genes exhibit different expression patterns in different tissues and are strongly induced under salt stress in wheat. The calcium-binding properties of the purified recombinant TaCRT1 protein were determined using a PIPES/Arsenazo III analysis. TaCRT1 gene overexpression in Nicotiana tabacum decreased salt stress damage in transgenic tobacco plants. Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions. Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.</description><subject>Abiotic stress</subject><subject>Analysis</subject><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>Bioinformatics</subject><subject>Calcium</subject><subject>Calreticulin</subject><subject>Calreticulin - genetics</subject><subject>Calreticulin - metabolism</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Cell adhesion &amp; migration</subject><subject>Chinese cabbage</subject><subject>Cloning, Molecular</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Genomics</subject><subject>Germplasm</subject><subject>Growth conditions</subject><subject>Homeostasis</subject><subject>Homology</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - physiology</subject><subject>Organ Specificity</subject><subject>Oryza</subject><subject>Peroxidase</subject><subject>Peroxidase - metabolism</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant sciences</subject><subject>Plant tissues</subject><subject>Plants (botany)</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - physiology</subject><subject>Proteins</subject><subject>Salinity</subject><subject>Salinity tolerance</subject><subject>Salts</subject><subject>Stress</subject><subject>Stress, Physiological</subject><subject>Stresses</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tobacco</subject><subject>Transgenic plants</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99r2zAQx83YWLts_8HYDIPRPiSTLFmyXgYl7EegEGjNXoUsnx0VxcokO7T__ZTFLfHow9CDxOlz39Od7pLkPUYLTDj-cucG3ym72LkOFghTlAv8IjnHgmRzliHy8uR8lrwJ4Q6hnBSMvU7OMkaZKHJxnrTrPXi433kIwbgudU2q0tKb3uhhmyoIvdnHw1JZDwebNV3aQgfpRamWNyW-TFfbnXd7COmtipemf0hLZ8GrTkMa4dJVSmv3NnnVKBvg3bjPkvL7t3L5c369_rFaXl3PNRNZPyeMZoLjSpOqqBFTwIWgimpe8CbLUZEBZzVA1VQkp5oVFDHRYJXF5JucIjJLPh5ld9YFOZYoSMyzjHKSCRKJ1ZGonbqTO2-2yj9Ip4z8a3C-lcrHTC1IopXKERW8QojWCImaE9XkGlcVAVoVUevrGG2otlBr6Hqv7ER0etOZjWzdXlKGOEU4ClyMAt79HmKx5dYEDdaqDtxwfLcgCMePmyWf_kGfz26kWhUTMF3jYlx9EJVXlGDGOcoOYRfPUHHVsDU69lNjon3icDlxiEwP932rhhDk6vbm_9n1ryn7-YTdgLL9Jjg79LEVwxSkR1B7F4KH5qnIGMnDODxWQx7GQY7jEN0-nH7Qk9Nj_5M_OLID8w</recordid><startdate>20151015</startdate><enddate>20151015</enddate><creator>Xiang, Yang</creator><creator>Lu, Yun Hai</creator><creator>Song, Min</creator><creator>Wang, Yun</creator><creator>Xu, Wenqi</creator><creator>Wu, Lintao</creator><creator>Wang, Hancheng</creator><creator>Ma, Zhengqiang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20151015</creationdate><title>Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco</title><author>Xiang, Yang ; Lu, Yun Hai ; Song, Min ; Wang, Yun ; Xu, Wenqi ; Wu, Lintao ; Wang, Hancheng ; Ma, Zhengqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-3642971bc3b8d06ae7994a4c787f25082e76deebfb354c684069f1a2059f5403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abiotic stress</topic><topic>Analysis</topic><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>Bioinformatics</topic><topic>Calcium</topic><topic>Calreticulin</topic><topic>Calreticulin - genetics</topic><topic>Calreticulin - metabolism</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Cell adhesion &amp; migration</topic><topic>Chinese cabbage</topic><topic>Cloning, Molecular</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic engineering</topic><topic>Genomics</topic><topic>Germplasm</topic><topic>Growth conditions</topic><topic>Homeostasis</topic><topic>Homology</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - physiology</topic><topic>Organ Specificity</topic><topic>Oryza</topic><topic>Peroxidase</topic><topic>Peroxidase - metabolism</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant sciences</topic><topic>Plant tissues</topic><topic>Plants (botany)</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - physiology</topic><topic>Proteins</topic><topic>Salinity</topic><topic>Salinity tolerance</topic><topic>Salts</topic><topic>Stress</topic><topic>Stress, Physiological</topic><topic>Stresses</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tobacco</topic><topic>Transgenic plants</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiang, Yang</creatorcontrib><creatorcontrib>Lu, Yun Hai</creatorcontrib><creatorcontrib>Song, Min</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><creatorcontrib>Xu, Wenqi</creatorcontrib><creatorcontrib>Wu, Lintao</creatorcontrib><creatorcontrib>Wang, Hancheng</creatorcontrib><creatorcontrib>Ma, Zhengqiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints in Context (Gale)</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiang, Yang</au><au>Lu, Yun Hai</au><au>Song, Min</au><au>Wang, Yun</au><au>Xu, Wenqi</au><au>Wu, Lintao</au><au>Wang, Hancheng</au><au>Ma, Zhengqiang</au><au>Zhang, Jin-Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-10-15</date><risdate>2015</risdate><volume>10</volume><issue>10</issue><spage>e0140591</spage><epage>e0140591</epage><pages>e0140591-e0140591</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Calreticulin (CRT) is a highly conserved and abundant multifunctional protein that is encoded by a small gene family and is often associated with abiotic/biotic stress responses in plants. However, the roles played by this protein in salt stress responses in wheat (Triticum aestivum) remain obscure. In this study, three TaCRT genes were identified in wheat and named TaCRT1, TaCRT2 and TaCRT3-1 based on their sequence characteristics and their high homology to other known CRT genes. Quantitative real-time PCR expression data revealed that these three genes exhibit different expression patterns in different tissues and are strongly induced under salt stress in wheat. The calcium-binding properties of the purified recombinant TaCRT1 protein were determined using a PIPES/Arsenazo III analysis. TaCRT1 gene overexpression in Nicotiana tabacum decreased salt stress damage in transgenic tobacco plants. Physiological measurements indicated that transgenic tobacco plants showed higher activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) than non-transgenic tobacco under normal growth conditions. Interestingly, overexpression of the entire TaCRT1 gene or of partial TaCRT1 segments resulted in significantly higher tolerance to salt stress in transgenic plants compared with their WT counterparts, thus revealing the essential role of the C-domain of TaCRT1 in countering salt stress in plants.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26469859</pmid><doi>10.1371/journal.pone.0140591</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2015-10, Vol.10 (10), p.e0140591-e0140591
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1722473293
source Publicly Available Content (ProQuest); PubMed Central
subjects Abiotic stress
Analysis
Arabidopsis
Arabidopsis thaliana
Bioinformatics
Calcium
Calreticulin
Calreticulin - genetics
Calreticulin - metabolism
Catalase
Catalase - metabolism
Cell adhesion & migration
Chinese cabbage
Cloning, Molecular
Gene expression
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Genes
Genetic aspects
Genetic engineering
Genomics
Germplasm
Growth conditions
Homeostasis
Homology
Kinases
Laboratories
Nicotiana - genetics
Nicotiana - physiology
Organ Specificity
Oryza
Peroxidase
Peroxidase - metabolism
Physiological aspects
Physiology
Plant Proteins - genetics
Plant Proteins - metabolism
Plant sciences
Plant tissues
Plants (botany)
Plants, Genetically Modified - genetics
Plants, Genetically Modified - physiology
Proteins
Salinity
Salinity tolerance
Salts
Stress
Stress, Physiological
Stresses
Superoxide dismutase
Superoxide Dismutase - metabolism
Tobacco
Transgenic plants
Triticum - genetics
Triticum - metabolism
Triticum aestivum
Wheat
title Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T17%3A23%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Overexpression%20of%20a%20Triticum%20aestivum%20Calreticulin%20gene%20(TaCRT1)%20Improves%20Salinity%20Tolerance%20in%20Tobacco&rft.jtitle=PloS%20one&rft.au=Xiang,%20Yang&rft.date=2015-10-15&rft.volume=10&rft.issue=10&rft.spage=e0140591&rft.epage=e0140591&rft.pages=e0140591-e0140591&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0140591&rft_dat=%3Cgale_plos_%3EA431677021%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-3642971bc3b8d06ae7994a4c787f25082e76deebfb354c684069f1a2059f5403%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1722473293&rft_id=info:pmid/26469859&rft_galeid=A431677021&rfr_iscdi=true