Characterization of Bacterial Communities Associated with the Tyrian Purple Producing Gland in a Marine Gastropod

Dicathais orbita is a marine mollusc recognised for the production of anticancer compounds that are precursors to Tyrian purple. This study aimed to assess the diversity and identity of bacteria associated with the Tyrian purple producing hypobranchial gland, in comparison with foot tissue, using a...

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Bibliographic Details
Published in:PloS one 2015-10, Vol.10 (10), p.e0140725-e0140725
Main Authors: Ngangbam, Ajit Kumar, Baten, Abdul, Waters, Daniel L E, Whalan, Steve, Benkendorff, Kirsten
Format: Article
Language:English
Subjects:
Analysis
Animal taxonomy
Animals
Bacteria
Bacterial Typing Techniques
Bacteroidetes - genetics
Bacteroidetes - isolation & purification
Base Sequence
Biodiversity
Biosynthesis
Bromination
Cancer
Chemical properties
Community composition
Deoxyribonucleic acid
DNA
Engineering
Feet
Gastropoda - microbiology
Genetic aspects
Genetic testing
High-Throughput Nucleotide Sequencing
Indoles
Indoles - metabolism
Marine ecology
Microbiota - genetics
Mollusca
Mollusks
Multiculturalism & pluralism
Muricidae
Natural products
Neogastropoda
Next-generation sequencing
Phylogeny
Plant sciences
Proteobacteria - genetics
Proteobacteria - isolation & purification
Pseudomonas
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequence Analysis, DNA
Signal processing
Spirochaetales - genetics
Spirochaetales - isolation & purification
Streptomyces
Taxonomy
Tenericutes - genetics
Tenericutes - isolation & purification
Variable region
Waterborne diseases
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Summary:Dicathais orbita is a marine mollusc recognised for the production of anticancer compounds that are precursors to Tyrian purple. This study aimed to assess the diversity and identity of bacteria associated with the Tyrian purple producing hypobranchial gland, in comparison with foot tissue, using a high-throughput sequencing approach. Taxonomic and phylogenetic analysis of variable region V1-V3 of 16S rRNA bacterial gene amplicons in QIIME and MEGAN were carried out. This analysis revealed a highly diverse bacterial assemblage associated with the hypobranchial gland and foot tissues of D. orbita. The dominant bacterial phylum in the 16S rRNA bacterial profiling data set was Proteobacteria followed by Bacteroidetes, Tenericutes and Spirochaetes. In comparison to the foot, the hypobranchial gland had significantly lower bacterial diversity and a different community composition, based on taxonomic assignment at the genus level. A higher abundance of indole producing Vibrio spp. and the presence of bacteria with brominating capabilities in the hypobranchial gland suggest bacteria have a potential role in biosynthesis of Tyrian purple in D. orbita.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0140725