Metabolic Engineering of Klebsiella pneumoniae for the Production of 2-Butanone from Glucose

2-Butanone is an important commodity chemical of wide application in different areas. In this study, Klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversi...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-10, Vol.10 (10), p.e0140508-e0140508
Main Authors: Chen, Zhen, Sun, He, Huang, Jinhai, Wu, Yao, Liu, Dehua
Format: Article
Language:English
Subjects:
Biodiesel fuels
Biological activity
Biosynthetic Pathways
Butanediol
Butanones - metabolism
Catalysis
Chemical engineering
Chemistry
Cobamides - metabolism
Dehydration
Dehydrogenases
Deoxyribonucleic acid
DNA
E coli
Enzyme Activation
Enzymes
Escherichia coli
Fermentation
Gene Deletion
Gene Expression
Genes
Genetic aspects
Glucose
Glucose - metabolism
Glycerol
Hydro-Lyases - genetics
Hydro-Lyases - metabolism
Ketones
Kinetics
Klebsiella
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - metabolism
L-Lactate dehydrogenase
Lactate dehydrogenase
Lactic acid
LdhA gene
Metabolic engineering
Metabolic Engineering - methods
Metabolism
Metabolites
Microorganisms
Optimization
Physiological aspects
Plasmids
Production processes
Propanediol Dehydratase - genetics
Propanediol Dehydratase - metabolism
Saccharomyces cerevisiae
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:2-Butanone is an important commodity chemical of wide application in different areas. In this study, Klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. By introducing the diol dehydratase from Lactobacillus brevis and deleting the ldhA gene encoding lactate dehydrogenase, the engineered K. pneumoniae was able to accumulate 246 mg/L of 2-butanone in shake flask. With further optimization of culture condition, the titer of 2-butanone was increased to 450 mg/L. This study lays the basis for developing an efficient biological process for 2-butanone production.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0140508