Pharmacological Mechanisms of Cortical Enhancement Induced by the Repetitive Pairing of Visual/Cholinergic Stimulation

• Published in: PloS one 2015-10, Vol.10 (10), p.e0141663-e0141663
• Main Authors: Kang, Jun-Il, Huppé-Gourgues, Frédéric, Vaucher, Elvire
• Format: Article
• Language: English
• Subjects: Acetylcholine receptors (muscarinic); Acetylcholine receptors (nicotinic); Animals; Basal forebrain; Beta Rhythm - drug effects; Cholinergic Agents - pharmacology; Compounds; Deep Brain Stimulation; Electrical stimuli; Electrodes; Evoked Potentials, Visual; Experiments; Forebrain; Frequency dependence; GABA Agonists - pharmacology; GABA Antagonists - pharmacology; Gamma Rhythm - drug effects; Male; Neurons; Neurosciences; Orientation; Orientation behavior; Oscillations; Pharmacology; Photic Stimulation; Rats; Receptors; Receptors, Nicotinic - metabolism; Rodents; Sensitivity; Sensitivity enhancement; Stimulation; Training; Visual cortex; Visual Cortex - drug effects; Visual Cortex - physiology; Visual evoked potentials; Visual pathways; Visual stimuli; γ-Aminobutyric acid A receptors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0141663

Repetitive visual training paired with electrical activation of cholinergic projections to the primary visual cortex (V1) induces long-term enhancement of cortical processing in response to the visual training stimulus. To better determine the receptor subtypes mediating this effect the selective pharmacological blockade of V1 nicotinic (nAChR), M1 and M2 muscarinic (mAChR) or GABAergic A (GABAAR) receptors was performed during the training session and visual evoked potentials (VEPs) were recorded before and after training. The training session consisted of the exposure of awake, adult rats to an orientation-specific 0.12 CPD grating paired with an electrical stimulation of the basal forebrain for a duration of 1 week for 10 minutes per day. Pharmacological agents were infused intracortically during this period. The post-training VEP amplitude was significantly increased compared to the pre-training values for the trained spatial frequency and to adjacent spatial frequencies up to 0.3 CPD, suggesting a long-term increase of V1 sensitivity. This increase was totally blocked by the nAChR antagonist as well as by an M2 mAChR subtype and GABAAR antagonist. Moreover, administration of the M2 mAChR antagonist also significantly decreased the amplitude of the control VEPs, suggesting a suppressive effect on cortical responsiveness. However, the M1 mAChR antagonist blocked the increase of the VEP amplitude only for the high spatial frequency (0.3 CPD), suggesting that M1 role was limited to the spread of the enhancement effect to a higher spatial frequency. More generally, all the drugs used did block the VEP increase at 0.3 CPD. Further, use of each of the aforementioned receptor antagonists blocked training-induced changes in gamma and beta band oscillations. These findings demonstrate that visual training coupled with cholinergic stimulation improved perceptual sensitivity by enhancing cortical responsiveness in V1. This enhancement is mainly mediated by nAChRs, M2 mAChRs and GABAARs. The M1 mAChR subtype appears to be involved in spreading the enhancement of V1 cortical responsiveness to adjacent neurons.