Potential Response to Selection of HSP70 as a Component of Innate Immunity in the Abalone Haliotis rufescens

• Published in: PloS one 2015-11, Vol.10 (11), p.e0141959-e0141959
• Main Authors: Brokordt, Katherina B, González, Roxana C, Farías, William J, Winkler, Federico M
• Format: Article
• Language: English
• Subjects: Adults; Animals; Breeding; Care and treatment; Coefficient of variation; Development and progression; Disease; Disease resistance; Fish; Gastropoda - cytology; Gastropoda - genetics; Gastropoda - immunology; Gene expression; Genetic aspects; Genetic diversity; Genetic variance; Haliotis rufescens; Harvesting; Heat shock proteins; Hemocytes; Hemolymph; Hemolymph - cytology; Hemolymph - immunology; Heritability; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - immunology; Hsp70 protein; Immune response; Immune system; Immunity; Immunity, Innate - physiology; Immunostimulants; Immunostimulation; Innate immunity; Intracellular; Invertebrates; Microorganisms; Mollusca; Mollusks; Patient outcomes; Phenotypic variations; Proteins; Quantitative genetics; Red abalone; Serum levels; Young adults
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0141959

Assessing components of the immune system may reflect disease resistance. In some invertebrates, heat shock proteins (HSPs) are immune effectors and have been described as potent activators of the innate immune response. Several diseases have become a threat to abalone farming worldwide; therefore, increasing disease resistance is considered to be a long-term goal for breeding programs. A trait will respond to selection only if it is determined partially by additive genetic variation. The aim of this study was to estimate the heritability (h2) and the additive genetic coefficient of variation (CVA) of HSP70 as a component of innate immunity of the abalone Haliotis rufescens, in order to assess its potential response to selection. These genetic components were estimated for the variations in the intracellular (in haemocytes) and extracellular (serum) protein levels of HSP70 in response to an immunostimulant agent in 60 full-sib families of H. rufescens. Levels of HSP70 were measured twice in the same individuals, first when they were young and again when they were pre-harvest adults, to estimate the repeatability (R), the h2 and the potential response to selection of these traits at these life stages. High HSP70 levels were observed in abalones subjected to immunostimulation in both the intracellular and extracellular haemolymph fractions. This is the first time that changes in serum levels of HSP70 have been reported in response to an immune challenge in molluscs. HSP70 levels in both fractions and at both ages showed low h2 and R, with values that were not significantly different from zero. However, HSP70 induced levels had a CVA of 13.3-16.2% in young adults and of 2.7-8.1% in pre-harvest adults. Thus, despite its low h2, HSP70 synthesis in response to an immune challenge in red abalone has the potential to evolve through selection because of its large phenotypic variation and the presence of additive genetic variance, especially in young animals.