Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis

Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient...

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Published in:PloS one 2015-11, Vol.10 (11), p.e0142555-e0142555
Main Authors: Zhang, Yi, Xiao, Rong-Rong, Yin, Tailang, Zou, Wei, Tang, Yun, Ding, Jinli, Yang, Jing
Format: Article
Language:English
Subjects:
Analytical chemistry
Biology
Channels
Chemotaxis
Chemotaxis - drug effects
Chemotaxis - physiology
Concentration gradient
Conduction
Design
Education
Equipment Design
Finite Element Analysis
Fluid dynamics
Fluid flow
Fluorescein
High-Throughput Screening Assays - instrumentation
Humans
In Vitro Techniques
Lab-On-A-Chip Devices
Laboratories
Male
Mammals
Medicine
Methods
Microchannels
Microfluidic Analytical Techniques
Microfluidics
Observations
Progesterone
Progesterone - administration & dosage
Properties
Reproductive health
Silicon wafers
Sperm
Sperm Motility - drug effects
Sperm Motility - physiology
Spermatozoa
Spermatozoa - drug effects
Spermatozoa - physiology
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cited_by cdi_FETCH-LOGICAL-c4725-abed8fa08cd218bfb4fbcaddb285aff2abfe56f597da0b04cd8a6cbdea4bf1003
cites cdi_FETCH-LOGICAL-c4725-abed8fa08cd218bfb4fbcaddb285aff2abfe56f597da0b04cd8a6cbdea4bf1003
container_end_page e0142555
container_issue 11
container_start_page e0142555
container_title PloS one
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creator Zhang, Yi
Xiao, Rong-Rong
Yin, Tailang
Zou, Wei
Tang, Yun
Ding, Jinli
Yang, Jing
description Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient than traditional ones. In the present study, an easy-handle diffusion-based microfluidic chip was established. This device allowed for conduction of three parallel experiments on the same chip, and improved the performance of sperm chemotaxis research. In such a chip, there were six channels surrounding a hexagonal pool. The channels are connected to the hexagon by microchannels. Firstly, the fluid flow in the system was characterized; secondly, fluorescein solution was used to calibrate gradient profiles formed in the central hexagon; thirdly, sperm behavior was observed under two concentration gradients of progesterone (100 pM and 1 mM, respectively) as a validation of the device. Significant differences in chemotactic parameters were recognized between experimental and control groups (p < 0.05). Compared with control group, sperm motility was greatly enhanced in 1 mM group (p < 0.05), but no significant difference was found in 100 pM group. In conclusion, we proposed a microfluidic device for the study of sperm chemotaxis that was capable of generating multi-channel gradients on a chip and would help reduce experimental errors and save time in experiment.
doi_str_mv 10.1371/journal.pone.0142555
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However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient than traditional ones. In the present study, an easy-handle diffusion-based microfluidic chip was established. This device allowed for conduction of three parallel experiments on the same chip, and improved the performance of sperm chemotaxis research. In such a chip, there were six channels surrounding a hexagonal pool. The channels are connected to the hexagon by microchannels. Firstly, the fluid flow in the system was characterized; secondly, fluorescein solution was used to calibrate gradient profiles formed in the central hexagon; thirdly, sperm behavior was observed under two concentration gradients of progesterone (100 pM and 1 mM, respectively) as a validation of the device. Significant differences in chemotactic parameters were recognized between experimental and control groups (p &lt; 0.05). Compared with control group, sperm motility was greatly enhanced in 1 mM group (p &lt; 0.05), but no significant difference was found in 100 pM group. 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1932-6203
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subjects Analytical chemistry
Biology
Channels
Chemotaxis
Chemotaxis - drug effects
Chemotaxis - physiology
Concentration gradient
Conduction
Design
Education
Equipment Design
Finite Element Analysis
Fluid dynamics
Fluid flow
Fluorescein
High-Throughput Screening Assays - instrumentation
Humans
In Vitro Techniques
Lab-On-A-Chip Devices
Laboratories
Male
Mammals
Medicine
Methods
Microchannels
Microfluidic Analytical Techniques
Microfluidics
Observations
Progesterone
Progesterone - administration & dosage
Properties
Reproductive health
Silicon wafers
Sperm
Sperm Motility - drug effects
Sperm Motility - physiology
Spermatozoa
Spermatozoa - drug effects
Spermatozoa - physiology
title Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis
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