Loading…
Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis
Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient...
Saved in:
Published in: | PloS one 2015-11, Vol.10 (11), p.e0142555-e0142555 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c6075-f50ac37b5ee48eb929feec10fd98a92a92129a6e09c751a1b0a6866f762d6a313 |
---|---|
cites | cdi_FETCH-LOGICAL-c6075-f50ac37b5ee48eb929feec10fd98a92a92129a6e09c751a1b0a6866f762d6a313 |
container_end_page | e0142555 |
container_issue | 11 |
container_start_page | e0142555 |
container_title | PloS one |
container_volume | 10 |
creator | Zhang, Yi Xiao, Rong-Rong Yin, Tailang Zou, Wei Tang, Yun Ding, Jinli Yang, Jing |
description | Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient than traditional ones. In the present study, an easy-handle diffusion-based microfluidic chip was established. This device allowed for conduction of three parallel experiments on the same chip, and improved the performance of sperm chemotaxis research. In such a chip, there were six channels surrounding a hexagonal pool. The channels are connected to the hexagon by microchannels. Firstly, the fluid flow in the system was characterized; secondly, fluorescein solution was used to calibrate gradient profiles formed in the central hexagon; thirdly, sperm behavior was observed under two concentration gradients of progesterone (100 pM and 1 mM, respectively) as a validation of the device. Significant differences in chemotactic parameters were recognized between experimental and control groups (p < 0.05). Compared with control group, sperm motility was greatly enhanced in 1 mM group (p < 0.05), but no significant difference was found in 100 pM group. In conclusion, we proposed a microfluidic device for the study of sperm chemotaxis that was capable of generating multi-channel gradients on a chip and would help reduce experimental errors and save time in experiment. |
doi_str_mv | 10.1371/journal.pone.0142555 |
format | article |
fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1732311979</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A434196723</galeid><doaj_id>oai_doaj_org_article_716cc8e19584424d9a5e7f4db751bd9d</doaj_id><sourcerecordid>A434196723</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6075-f50ac37b5ee48eb929feec10fd98a92a92129a6e09c751a1b0a6866f762d6a313</originalsourceid><addsrcrecordid>eNqNk1Fv0zAQxyMEYmPwDRBEQkLw0GLHTlLzgDQN6CoNTWKFV-viXBJXSdzZSRl8etw2qxq0B5RISezf_c_3z10QvKRkSllKP6xMb1uop2vT4pRQHsVx_Cg4pYJFkyQi7PHR-0nwzLkVITGbJcnT4CRKPCw4PQ3sHFu00GnThqYI5xZyjW3nQv8N4TetrCnqXudahZ9xoxV-DJfmF9jc717qsposK2v6slr3XbhoN-g6XR7UbtZoG-jMHwPhRYWN6eBOu-fBkwJqhy-G51nw4-uX5cXl5Op6vrg4v5qohKTxpIgJKJZmMSKfYSYiUSAqSopczEBE_qaRgASJUGlMgWYEEl9dkSZRngCj7Cx4vddd18bJwS4nacoiRqlIhScWeyI3sJJrqxuwv6UBLXcLxpYSbKdVjTKliVIzpCKecR7xXECMacHzzOfOcpF7rU9Dtj5rMFfeRAv1SHS80-pKlmYjecJJvDvMu0HAmtveGykb7RTWNbRo-t25GRURJ9yjb_5BH65uoErwBei2MD6v2orKc844FUkaMU9NH6D8lWOjle-tQvv1UcD7UYBnOrzrSuidk4ub7__PXv8cs2-P2Aqh7ipn6n7bTG4M8j3oe9M5i8XBZErkdjTu3ZDb0ZDDaPiwV8c_6BB0PwvsL80nCYc</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1732311979</pqid></control><display><type>article</type><title>Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Zhang, Yi ; Xiao, Rong-Rong ; Yin, Tailang ; Zou, Wei ; Tang, Yun ; Ding, Jinli ; Yang, Jing</creator><contributor>Wood, Chris D</contributor><creatorcontrib>Zhang, Yi ; Xiao, Rong-Rong ; Yin, Tailang ; Zou, Wei ; Tang, Yun ; Ding, Jinli ; Yang, Jing ; Wood, Chris D</creatorcontrib><description>Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient than traditional ones. In the present study, an easy-handle diffusion-based microfluidic chip was established. This device allowed for conduction of three parallel experiments on the same chip, and improved the performance of sperm chemotaxis research. In such a chip, there were six channels surrounding a hexagonal pool. The channels are connected to the hexagon by microchannels. Firstly, the fluid flow in the system was characterized; secondly, fluorescein solution was used to calibrate gradient profiles formed in the central hexagon; thirdly, sperm behavior was observed under two concentration gradients of progesterone (100 pM and 1 mM, respectively) as a validation of the device. Significant differences in chemotactic parameters were recognized between experimental and control groups (p < 0.05). Compared with control group, sperm motility was greatly enhanced in 1 mM group (p < 0.05), but no significant difference was found in 100 pM group. In conclusion, we proposed a microfluidic device for the study of sperm chemotaxis that was capable of generating multi-channel gradients on a chip and would help reduce experimental errors and save time in experiment.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0142555</identifier><identifier>PMID: 26555941</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analytical chemistry ; Biology ; Channels ; Chemotaxis ; Chemotaxis - drug effects ; Chemotaxis - physiology ; Concentration gradient ; Conduction ; Design ; Education ; Equipment Design ; Finite Element Analysis ; Fluid dynamics ; Fluid flow ; Fluorescein ; High-Throughput Screening Assays - instrumentation ; Humans ; In Vitro Techniques ; Lab-On-A-Chip Devices ; Laboratories ; Male ; Mammals ; Medicine ; Methods ; Microchannels ; Microfluidic Analytical Techniques ; Microfluidics ; Observations ; Progesterone ; Progesterone - administration & dosage ; Properties ; Reproductive health ; Silicon wafers ; Sperm ; Sperm Motility - drug effects ; Sperm Motility - physiology ; Spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - physiology</subject><ispartof>PloS one, 2015-11, Vol.10 (11), p.e0142555-e0142555</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Zhang et al 2015 Zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6075-f50ac37b5ee48eb929feec10fd98a92a92129a6e09c751a1b0a6866f762d6a313</citedby><cites>FETCH-LOGICAL-c6075-f50ac37b5ee48eb929feec10fd98a92a92129a6e09c751a1b0a6866f762d6a313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1732311979/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1732311979?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26555941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wood, Chris D</contributor><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Xiao, Rong-Rong</creatorcontrib><creatorcontrib>Yin, Tailang</creatorcontrib><creatorcontrib>Zou, Wei</creatorcontrib><creatorcontrib>Tang, Yun</creatorcontrib><creatorcontrib>Ding, Jinli</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><title>Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient than traditional ones. In the present study, an easy-handle diffusion-based microfluidic chip was established. This device allowed for conduction of three parallel experiments on the same chip, and improved the performance of sperm chemotaxis research. In such a chip, there were six channels surrounding a hexagonal pool. The channels are connected to the hexagon by microchannels. Firstly, the fluid flow in the system was characterized; secondly, fluorescein solution was used to calibrate gradient profiles formed in the central hexagon; thirdly, sperm behavior was observed under two concentration gradients of progesterone (100 pM and 1 mM, respectively) as a validation of the device. Significant differences in chemotactic parameters were recognized between experimental and control groups (p < 0.05). Compared with control group, sperm motility was greatly enhanced in 1 mM group (p < 0.05), but no significant difference was found in 100 pM group. In conclusion, we proposed a microfluidic device for the study of sperm chemotaxis that was capable of generating multi-channel gradients on a chip and would help reduce experimental errors and save time in experiment.</description><subject>Analytical chemistry</subject><subject>Biology</subject><subject>Channels</subject><subject>Chemotaxis</subject><subject>Chemotaxis - drug effects</subject><subject>Chemotaxis - physiology</subject><subject>Concentration gradient</subject><subject>Conduction</subject><subject>Design</subject><subject>Education</subject><subject>Equipment Design</subject><subject>Finite Element Analysis</subject><subject>Fluid dynamics</subject><subject>Fluid flow</subject><subject>Fluorescein</subject><subject>High-Throughput Screening Assays - instrumentation</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Lab-On-A-Chip Devices</subject><subject>Laboratories</subject><subject>Male</subject><subject>Mammals</subject><subject>Medicine</subject><subject>Methods</subject><subject>Microchannels</subject><subject>Microfluidic Analytical Techniques</subject><subject>Microfluidics</subject><subject>Observations</subject><subject>Progesterone</subject><subject>Progesterone - administration & dosage</subject><subject>Properties</subject><subject>Reproductive health</subject><subject>Silicon wafers</subject><subject>Sperm</subject><subject>Sperm Motility - drug effects</subject><subject>Sperm Motility - physiology</subject><subject>Spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1Fv0zAQxyMEYmPwDRBEQkLw0GLHTlLzgDQN6CoNTWKFV-viXBJXSdzZSRl8etw2qxq0B5RISezf_c_3z10QvKRkSllKP6xMb1uop2vT4pRQHsVx_Cg4pYJFkyQi7PHR-0nwzLkVITGbJcnT4CRKPCw4PQ3sHFu00GnThqYI5xZyjW3nQv8N4TetrCnqXudahZ9xoxV-DJfmF9jc717qsposK2v6slr3XbhoN-g6XR7UbtZoG-jMHwPhRYWN6eBOu-fBkwJqhy-G51nw4-uX5cXl5Op6vrg4v5qohKTxpIgJKJZmMSKfYSYiUSAqSopczEBE_qaRgASJUGlMgWYEEl9dkSZRngCj7Cx4vddd18bJwS4nacoiRqlIhScWeyI3sJJrqxuwv6UBLXcLxpYSbKdVjTKliVIzpCKecR7xXECMacHzzOfOcpF7rU9Dtj5rMFfeRAv1SHS80-pKlmYjecJJvDvMu0HAmtveGykb7RTWNbRo-t25GRURJ9yjb_5BH65uoErwBei2MD6v2orKc844FUkaMU9NH6D8lWOjle-tQvv1UcD7UYBnOrzrSuidk4ub7__PXv8cs2-P2Aqh7ipn6n7bTG4M8j3oe9M5i8XBZErkdjTu3ZDb0ZDDaPiwV8c_6BB0PwvsL80nCYc</recordid><startdate>20151110</startdate><enddate>20151110</enddate><creator>Zhang, Yi</creator><creator>Xiao, Rong-Rong</creator><creator>Yin, Tailang</creator><creator>Zou, Wei</creator><creator>Tang, Yun</creator><creator>Ding, Jinli</creator><creator>Yang, Jing</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20151110</creationdate><title>Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis</title><author>Zhang, Yi ; Xiao, Rong-Rong ; Yin, Tailang ; Zou, Wei ; Tang, Yun ; Ding, Jinli ; Yang, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6075-f50ac37b5ee48eb929feec10fd98a92a92129a6e09c751a1b0a6866f762d6a313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analytical chemistry</topic><topic>Biology</topic><topic>Channels</topic><topic>Chemotaxis</topic><topic>Chemotaxis - drug effects</topic><topic>Chemotaxis - physiology</topic><topic>Concentration gradient</topic><topic>Conduction</topic><topic>Design</topic><topic>Education</topic><topic>Equipment Design</topic><topic>Finite Element Analysis</topic><topic>Fluid dynamics</topic><topic>Fluid flow</topic><topic>Fluorescein</topic><topic>High-Throughput Screening Assays - instrumentation</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Lab-On-A-Chip Devices</topic><topic>Laboratories</topic><topic>Male</topic><topic>Mammals</topic><topic>Medicine</topic><topic>Methods</topic><topic>Microchannels</topic><topic>Microfluidic Analytical Techniques</topic><topic>Microfluidics</topic><topic>Observations</topic><topic>Progesterone</topic><topic>Progesterone - administration & dosage</topic><topic>Properties</topic><topic>Reproductive health</topic><topic>Silicon wafers</topic><topic>Sperm</topic><topic>Sperm Motility - drug effects</topic><topic>Sperm Motility - physiology</topic><topic>Spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Xiao, Rong-Rong</creatorcontrib><creatorcontrib>Yin, Tailang</creatorcontrib><creatorcontrib>Zou, Wei</creatorcontrib><creatorcontrib>Tang, Yun</creatorcontrib><creatorcontrib>Ding, Jinli</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale_Opposing Viewpoints In Context</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yi</au><au>Xiao, Rong-Rong</au><au>Yin, Tailang</au><au>Zou, Wei</au><au>Tang, Yun</au><au>Ding, Jinli</au><au>Yang, Jing</au><au>Wood, Chris D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-11-10</date><risdate>2015</risdate><volume>10</volume><issue>11</issue><spage>e0142555</spage><epage>e0142555</epage><pages>e0142555-e0142555</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Various research tools have been used for in vitro detection of sperm chemotaxis. However, they are typically poor in maintenance of gradient stability, not to mention their low efficiency. Microfluidic device offers a new experimental platform for better control over chemical concentration gradient than traditional ones. In the present study, an easy-handle diffusion-based microfluidic chip was established. This device allowed for conduction of three parallel experiments on the same chip, and improved the performance of sperm chemotaxis research. In such a chip, there were six channels surrounding a hexagonal pool. The channels are connected to the hexagon by microchannels. Firstly, the fluid flow in the system was characterized; secondly, fluorescein solution was used to calibrate gradient profiles formed in the central hexagon; thirdly, sperm behavior was observed under two concentration gradients of progesterone (100 pM and 1 mM, respectively) as a validation of the device. Significant differences in chemotactic parameters were recognized between experimental and control groups (p < 0.05). Compared with control group, sperm motility was greatly enhanced in 1 mM group (p < 0.05), but no significant difference was found in 100 pM group. In conclusion, we proposed a microfluidic device for the study of sperm chemotaxis that was capable of generating multi-channel gradients on a chip and would help reduce experimental errors and save time in experiment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26555941</pmid><doi>10.1371/journal.pone.0142555</doi><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2015-11, Vol.10 (11), p.e0142555-e0142555 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_1732311979 |
source | Publicly Available Content Database; PubMed Central |
subjects | Analytical chemistry Biology Channels Chemotaxis Chemotaxis - drug effects Chemotaxis - physiology Concentration gradient Conduction Design Education Equipment Design Finite Element Analysis Fluid dynamics Fluid flow Fluorescein High-Throughput Screening Assays - instrumentation Humans In Vitro Techniques Lab-On-A-Chip Devices Laboratories Male Mammals Medicine Methods Microchannels Microfluidic Analytical Techniques Microfluidics Observations Progesterone Progesterone - administration & dosage Properties Reproductive health Silicon wafers Sperm Sperm Motility - drug effects Sperm Motility - physiology Spermatozoa Spermatozoa - drug effects Spermatozoa - physiology |
title | Generation of Gradients on a Microfluidic Device: Toward a High-Throughput Investigation of Spermatozoa Chemotaxis |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T13%3A45%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Generation%20of%20Gradients%20on%20a%20Microfluidic%20Device:%20Toward%20a%20High-Throughput%20Investigation%20of%20Spermatozoa%20Chemotaxis&rft.jtitle=PloS%20one&rft.au=Zhang,%20Yi&rft.date=2015-11-10&rft.volume=10&rft.issue=11&rft.spage=e0142555&rft.epage=e0142555&rft.pages=e0142555-e0142555&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0142555&rft_dat=%3Cgale_plos_%3EA434196723%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c6075-f50ac37b5ee48eb929feec10fd98a92a92129a6e09c751a1b0a6866f762d6a313%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1732311979&rft_id=info:pmid/26555941&rft_galeid=A434196723&rfr_iscdi=true |