Photographic-Based Optical Evaluation of Tissues and Biomaterials Used for Corneal Surface Repair: A New Easy-Applied Method

Tissues and biomaterials used for corneal surface repair require fulfilling specific optical standards prior to implantation in the patient. However, there is not a feasible evaluation method to be applied in clinical or Good Manufacturing Practice settings. In this study, we describe and assess an...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-11, Vol.10 (11), p.e0142099-e0142099
Main Authors: Gonzalez-Andrades, Miguel, Cardona, Juan de la Cruz, Ionescu, Ana Maria, Mosse, Charles A, Brown, Robert A
Format: Article
Language:English
Subjects:
Amnion
Amnion - chemistry
Animals
Biocompatible Materials - chemistry
Biological products
Biomaterials
Biomedical materials
Blurring
Collagen
Collagen - chemistry
Cornea
Cornea - physiology
Corneal Transplantation
Correlation analysis
Equipment Design
Evaluation
Feasibility studies
Good Manufacturing Practice
Grafts
Humans
Implantation
Laboratories
Lasers
Light
Membranes
Methods
Optical properties
Optics
Photography - instrumentation
Photography - methods
Photometry - instrumentation
Photometry - methods
Plastics
Reflectance
Regeneration
Repair
Retina
Scaffolds
Surgical implants
Tissue engineering
Tissue Scaffolds - chemistry
Transmittance
Transparency
Transplants & implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tissues and biomaterials used for corneal surface repair require fulfilling specific optical standards prior to implantation in the patient. However, there is not a feasible evaluation method to be applied in clinical or Good Manufacturing Practice settings. In this study, we describe and assess an innovative easy-applied photographic-based method (PBM) for measuring functional optical blurring and transparency in corneal surface grafts. Plastic compressed collagen scaffolds (PCCS) and multilayered amniotic membranes (AM) samples were optically and histologically evaluated. Transparency and image blurring measures were obtained by PBM, analyzing photographic images of a standardized band pattern taken through the samples. These measures were compared and correlated to those obtained applying the Inverse Adding-Doubling (IAD) technique, which is the gold standard method. All the samples used for optical evaluation by PBM or IAD were histological suitable. PCCS samples presented transmittance values higher than 60%, values that increased with increasing wavelength as determined by IAD. The PBM indicated that PCCS had a transparency ratio (TR) value of 80.3 ± 2.8%, with a blurring index (BI) of 50.6 ± 4.2%. TR and BI obtained from the PBM showed a high correlation (ρ>|0.6|) with the diffuse transmittance and the diffuse reflectance, both determined using the IAD (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0142099