Zebrafish Egg Infection Model for Studying Candida albicans Adhesion Factors

Disseminated candidiasis is associated with 30-40% mortality in severely immunocompromised patients. Among the causal agents, Candida albicans is the dominant one. Various animal models have been developed for investigating gene functions in C. albicans. Zebrafish injection models have increasingly...

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Bibliographic Details
Published in:PloS one 2015-11, Vol.10 (11), p.e0143048-e0143048
Main Authors: Chen, Yin-Zhi, Yang, Yun-Liang, Chu, Wen-Li, You, May-Su, Lo, Hsiu-Jung
Format: Article
Language:English
Subjects:
Animal models
Animals
Biofilms
Candida
Candida albicans
Candida albicans - physiology
Candidiasis
Candidiasis - microbiology
Care and treatment
Clonal deletion
Danio rerio
Development and progression
Embryo, Nonmammalian - microbiology
Embryos
Fecundity
Genetic aspects
Health aspects
Hyphae - physiology
Immunity
Immunocompromised hosts
Infections
Mortality
Mutation
Mycoses
Ovum - microbiology
Pathogenesis
Tissue Culture Techniques
Virulence
Virulence (Microbiology)
Yeast
Zebrafish
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Summary:Disseminated candidiasis is associated with 30-40% mortality in severely immunocompromised patients. Among the causal agents, Candida albicans is the dominant one. Various animal models have been developed for investigating gene functions in C. albicans. Zebrafish injection models have increasingly been applied in elucidating C. albicans pathogenesis because of the conserved immunity, prolific fecundity of the zebrafish and the low costs of care systems. In this study, we established a simple, noninvasive zebrafish egg bath infection model, defined its optimal conditions, and evaluated the model with various C. albicans mutant strains. The deletion of SAP6 did not have significant effect on the virulence. By contrast, the deletion of BCR1, CPH1, EFG1, or TEC1 significantly reduced the virulence under current conditions. Furthermore, all embryos survived when co-incubated with bcr1/bcr1, cph1/cph1 efg1/efg1, efg1/efg1, or tec1/tec1 mutant cells. The results indicated that our novel zebrafish model is time-saving and cost effective.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0143048