454 Pyrosequencing of Olive (Olea europaea L.) Transcriptome in Response to Salinity

• Published in: PloS one 2015-11, Vol.10 (11), p.e0143000-e0143000
• Main Authors: Bazakos, Christos, Manioudaki, Maria E, Sarropoulou, Elena, Spano, Thodhoraq, Kalaitzis, Panagiotis
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Arabidopsis; Arabidopsis thaliana; Base Sequence; Biotechnology; Cluster Analysis; Clusters; Cultivars; Cultivation; Fruit trees; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Gene Library; Gene Ontology; Gene sequencing; Genes; Genetics; High-Throughput Nucleotide Sequencing - methods; Horticulture; Ion Transport - drug effects; Ion Transport - genetics; Leaves; Molecular Sequence Annotation; Olea - drug effects; Olea - genetics; Olea europaea; Physiology; Plant Leaves - drug effects; Plant Leaves - genetics; Plant Roots - drug effects; Plant Roots - genetics; Quality control; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Roots; Saline water; Salinity; Salinity effects; Salt; Salt tolerance; Salts; Signal transduction; Sodium Chloride - pharmacology; Stress, Physiological - drug effects; Stress, Physiological - genetics; Studies; Technology; Technology utilization; Transcription factors; Transcription Factors - metabolism; Transcriptome - drug effects; Transcriptome - genetics; Water quality
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0143000

Olive (Olea europaea L.) is one of the most important crops in the Mediterranean region. The expansion of cultivation in areas irrigated with low quality and saline water has negative effects on growth and productivity however the investigation of the molecular basis of salt tolerance in olive trees has been only recently initiated. To this end, we investigated the molecular response of cultivar Kalamon to salinity stress using next-generation sequencing technology to explore the transcriptome profile of olive leaves and roots and identify differentially expressed genes that are related to salt tolerance response. Out of 291,958 obtained trimmed reads, 28,270 unique transcripts were identified of which 35% are annotated, a percentage that is comparable to similar reports on non-model plants. Among the 1,624 clusters in roots that comprise more than one read, 24 were differentially expressed comprising 9 down- and 15 up-regulated genes. Respectively, inleaves, among the 2,642 clusters, 70 were identified as differentially expressed, with 14 down- and 56 up-regulated genes. Using next-generation sequencing technology we were able to identify salt-response-related transcripts. Furthermore we provide an annotated transcriptome of olive as well as expression data, which are both significant tools for further molecular studies in olive.