Tie2-Expressing Monocytes Are Associated with Identification and Prognoses of Hepatitis B Virus Related Hepatocellular Carcinoma after Resection

• Published in: PloS one 2015-11, Vol.10 (11), p.e0143657-e0143657
• Main Authors: He, Yi-Feng, Wang, Chao-Qun, Yu, Yao, Qian, Jing, Song, Kang, Sun, Qi-Man, Zhou, Jian
• Format: Article
• Language: English
• Subjects: Adult; Aged; Angiogenesis; Angiopoietin; Benign; Biomarkers; Blood; Blood vessels; Breast cancer; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cytometry; Education; Enzyme-linked immunosorbent assay; Female; Flow cytometry; Growth factors; Hepatectomy; Hepatitis; Hepatitis B; Hepatitis B virus - pathogenicity; Hepatocellular carcinoma; Hepatology; Hospitals; Humans; Immunofluorescence; Immunoglobulins; Immunology; Laboratories; Liver; Liver cancer; Liver cirrhosis; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Male; Medical prognosis; Medical research; Middle Aged; Monocytes; Monocytes - metabolism; Neoplasm Recurrence, Local - metabolism; Neovascularization, Pathologic - metabolism; Neovascularization, Pathologic - pathology; Patients; Peripheral blood; Prognosis; Receptor, TIE-2 - metabolism; Staining; Surgery; Tumors; Viruses; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0143657

Tie2-expressing monocytes (TEMs) are found in various tumors, involved in forming tumor blood vessels and expressing several important proangiogenic factors. The goals of this study were to evaluate the value of TEMs in diagnosing and predicting the prognosis of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Flow cytometry was performed to identify and count TEMs in peripheral blood monocytes from HCC patients (n = 84) receiving hepatectomy, HBV cirrhotic patients (n = 21), benign tumors patients (n = 15) and healthy volunteers (n = 23). Angiopoietin-2 (Ang-2) levels in the plasma were determined by enzyme linked immunosorbent assay. The distribution of TEMs in tumor tissue was observed by immunofluorescence staining. Then we determined the vascular area as a percentage of tumor area (vascular area/tumor area) by immunohistochemical staining. Finally the prognostic significance of TEMs and other clinicopathologic factors was evaluated. Percentage of TEMs in peripheral blood monocytes significantly increased in HCC patients compared with HBV cirrhotic patients and healthy donors (both P< 0.001). However there was no significance in benign liver tumor (P = 0.482). In addition, the percentage of circulating TEMs was positively correlated with plasma Ang-2 concentration (P