Nestin Positive Bone Marrow Derived Cells Responded to Injury Mobilize into Peripheral Circulation and Participate in Skin Defect Healing

• Published in: PloS one 2015-12, Vol.10 (12), p.e0143368-e0143368
• Main Authors: Yang, Yi, Pang, Danlin, Hu, Chenghu, Lv, Yajie, He, Tao, An, Yulin, Tang, Zhangui, Deng, Zhihong
• Format: Article
• Language: English
• Subjects: Animals; Blood; Bone marrow; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Cancer; Cell Movement - drug effects; Cell Movement - physiology; Cerebrospinal fluid; Colony-stimulating factor; Fluorescence; Gene expression; Genetic aspects; Granulocyte colony-stimulating factor; Granulocyte Colony-Stimulating Factor - pharmacology; Granulocytes; Green fluorescent protein; Heart attacks; In vivo methods and tests; Injuries; Laboratory animals; Leukocytes (granulocytic); Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Mice; Nestin; Nestin - metabolism; Organs; Otolaryngology; Peripheral blood; Peripheral circulation; Physiological aspects; Repair; Skin; Skin injuries; Stem cell transplantation; Stem cells; Studies; Tissue engineering; Transplants & implants; Wound healing; Wound Healing - drug effects; Wound Healing - physiology; Wounds
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0143368

Exogenously infused mesenchymal stem cells (MSCs) are thought to migrate to injury site through peripheral blood stream and participate in tissue repair. However, whether and how endogenous bone marrow MSCs mobilized to circulating and targeted to tissue injury has raised some controversy, and related studies were restricted by the difficulty of MSCs identifying in vivo. Nestin, a kind of intermediate filament protein initially identified in neuroepithelial stem cells, was recently reported as a credible criteria for MSCs in bone marrow. In this study, we used a green fluorescent protein (GFP) labeled bone marrow replacement model to trace the nestin positive bone marrow derived cells (BMDCs) of skin defected-mice. We found that after skin injured, numbers of nestin+ cells in peripheral blood and bone marrow both increased. A remarkable concentration of nestin+ BMDCs around skin wound was detected, while few of these cells could be observed in uninjured skin or other organs. This recruitment effect could not be promoted by granulocyte colony-stimulating factor (G-CSF), suggests a different mobilization mechanism from ones G-CSF takes effect on hematopoietic cells. Our results proposed nestin+ BMDCs as mobilized candidates in skin injury repair, which provide a new insight of endogenous MSCs therapy.