Endoplasmic Reticulum Stress in Heat- and Shake-Induced Injury in the Rat Small Intestine

• Published in: PloS one 2015-12, Vol.10 (12), p.e0143922-e0143922
• Main Authors: Yin, Peng, Xu, Jianqin, He, Shasha, Liu, Fenghua, Yin, Jie, Wan, Changrong, Mei, Chen, Yin, Yulong, Xu, Xiaolong, Xia, Zhaofei
• Format: Article
• Language: English
• Subjects: AKT protein; Animals; Apoptosis; Autophagy; Caspase 12 - biosynthesis; Caspase 3 - biosynthesis; Cell death; Damage; DNA microarrays; Encyclopedias; Endoplasmic reticulum; Endoplasmic Reticulum Stress; Epithelial cells; Gene expression; Gene Expression Profiling; Gene Expression Regulation; Genes; Genomes; Heat; Heat Exhaustion - metabolism; Heat Exhaustion - pathology; Injuries; Intestine, Small - metabolism; Intestine, Small - pathology; Jejunum; Kinases; Lamina propria; Male; MAP kinase; MAP Kinase Signaling System; Microtubule-Associated Proteins - biosynthesis; Oligonucleotide Array Sequence Analysis; Paraffin; Phagocytosis; Phosphorylation; Photomicrographs; Physiological aspects; Protein kinase; Proteins; Proto-Oncogene Proteins c-akt - biosynthesis; Rapamycin; Rats; Rats, Sprague-Dawley; Rodents; Shedding; Signal transduction; Signaling; Small intestine; Stress; Stresses; TOR protein; TOR Serine-Threonine Kinases - biosynthesis; Veterinary colleges; Vibration - adverse effects; Villus
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0143922

We investigated the mechanisms underlying damage to rat small intestine in heat- and shake-induced stress. Eighteen Sprague-Dawley rats were randomly divided into a control group and a 3-day stressed group treated 2 h daily for 3 days on a rotary platform at 35°C and 60 r/min. Hematoxylin and eosin-stained paraffin sections of the jejunum following stress revealed shedding of the villus tip epithelial cells and lamina propria exposure. Apoptosis increased at the villus tip and extended to the basement membrane. Photomicrographs revealed that the microvilli were shorter and sparser; the nuclear envelope invaginated and gaps in the karyolemma increased; and the endoplasmic reticulum (ER) swelled significantly. Gene microarray analysis assessed 93 differentially expressed genes associated with apoptosis, ER stress, and autophagy. Relevant genes were compiled from the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Forty-one genes were involved in the regulation of apoptosis, fifteen were related to autophagy, and eleven responded to ER stress. According to KEGG, the apoptosis pathways, mitogen-activated protein kinase(MAPK) signaling pathway, the mammalian target of rapamycin (mTOR) signaling pathway, and regulation of autophagy were involved. Caspase3 (Casp3), caspase12 (Casp12), and microtubule-associate proteins 1 light chain 3(LC3) increased significantly at the villus tip while mTOR decreased; phosphorylated-AKT (P-AKT) decreased. ER stress was involved and induced autophagy and apoptosis in rat intestinal damage following heat and shake stress. Bioinformatic analysis will help determine the underlying mechanisms in stress-induced damage in the small intestine.