Complement Effectors of Inflammation in Cystic Fibrosis Lung Fluid Correlate with Clinical Measures of Disease

In cystic fibrosis (CF), lung damage is mediated by a cycle of obstruction, infection, and inflammation. Here we explored complement inflammatory effectors in CF lung fluid. In this study soluble fractions (sols) from sputum samples of 15 CF patients were assayed for complement effectors and analyze...

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Bibliographic Details
Published in:PloS one 2015-12, Vol.10 (12), p.e0144723-e0144723
Main Authors: Sass, Laura A, Hair, Pamela S, Perkins, Amy M, Shah, Tushar A, Krishna, Neel K, Cunnion, Kenji M
Format: Article
Language:English
Subjects:
Airway management
Bacteria
Body Fluids - immunology
Body mass
Body mass index
Body size
Care and treatment
Case-Control Studies
Child
Children
Children & youth
Complement
Complement C5a - metabolism
Complement component C1
Complement component C5a
Complications and side effects
Correlation analysis
Cystic fibrosis
Cystic Fibrosis - immunology
Cystic Fibrosis - physiopathology
Effectors
Fluids
Follow-Up Studies
Hair
Health aspects
Hospitals
Humans
Incubation
Inflammation
Inflammation - immunology
Inflammation - physiopathology
Lectins
Lung - immunology
Lung - physiopathology
Lung diseases
Lungs
Medical schools
Neutrophils
Pathogens
Pediatrics
Peptide inhibitors
Prognosis
Proteins
Pseudomonas aeruginosa
Pseudomonas Infections - immunology
Pseudomonas Infections - microbiology
Pseudomonas Infections - physiopathology
Risk factors
Sputum
Sputum - immunology
Staphylococcus aureus
Staphylococcus aureus - immunology
Transplants & implants
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Summary:In cystic fibrosis (CF), lung damage is mediated by a cycle of obstruction, infection, and inflammation. Here we explored complement inflammatory effectors in CF lung fluid. In this study soluble fractions (sols) from sputum samples of 15 CF patients were assayed for complement effectors and analyzed with clinical measurements. The pro-inflammatory peptide C5a was increased 4.8-fold (P = 0.04) in CF sols compared with controls. Incubation of CF sols with P. aeruginosa or S. aureus increased C5a concentration 2.3-fold (P = 0.02). A peptide inhibitor of complement C1 (PIC1) completely blocked the increase in C5a concentration from P. aeruginosa in CF sol in vitro (P = 0.001). C5a concentration in CF sol correlated inversely with body mass index (BMI) percentile in children (r = -0.77, P = 0.04). C3a, which has anti-inflammatory effects, correlated positively with FEV1% predicted (rs = 0.63, P = 0.02). These results suggest that complement effectors may significantly impact inflammation in CF lung fluid.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0144723