Towards Prebiotic Catalytic Amyloids Using High Throughput Screening

• Published in: PloS one 2015-12, Vol.10 (12), p.e0143948-e0143948
• Main Authors: Friedmann, Michael P, Torbeev, Vladimir, Zelenay, Viviane, Sobol, Alexander, Greenwald, Jason, Riek, Roland
• Format: Article
• Language: English
• Subjects: Amino acids; Amyloid - chemistry; Catalysis; Catalysts; Catalytic activity; Circular Dichroism; E coli; Enzymes; Esterase; Extreme high temperatures; Glycoproteins; High temperature; High-throughput screening; High-Throughput Screening Assays - methods; Humans; Hydrolysis; Industrial applications; Laboratories; Libraries; Medical screening; Microscopy, Electron, Transmission; Peptide Fragments - chemistry; Peptides; Physical chemistry; Polypeptides; Prebiotics; Protein structure; Proteins; Publishing industry; Screening; Spectroscopy, Fourier Transform Infrared; Tertiary structure; β-Amyloid
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0143948

Enzymes are capable of directing complex stereospecific transformations and of accelerating reaction rates many orders of magnitude. As even the simplest known enzymes comprise thousands of atoms, the question arises as to how such exquisite catalysts evolved. A logical predecessor would be shorter peptides, but they lack the defined structure and size that are apparently necessary for enzyme functions. However, some very short peptides are able to assemble into amyloids, thereby forming a well-defined tertiary structure called the cross-β-sheet, which bestows unique properties upon the peptides. We have hypothesized that amyloids could have been the catalytically active precursor to modern enzymes. To test this hypothesis, we designed an amyloid peptide library that could be screened for catalytic activity. Our approach, amenable to high-throughput methodologies, allowed us to find several peptides and peptide mixtures that form amyloids with esterase activity. These results indicate that amyloids, with their stability in a wide range of conditions and their potential as catalysts with low sequence specificity, would indeed be fitting precursors to modern enzymes. Furthermore, our approach can be efficiently expanded upon in library size, screening conditions, and target activity to yield novel amyloid catalysts with potential applications in aqueous-organic mixtures, at high temperature and in other extreme conditions that could be advantageous for industrial applications.