Ammonium Chloride Ingestion Attenuates Exercise-Induced mRNA Levels in Human Muscle

Minimizing the decrease in intracellular pH during high-intensity exercise training promotes greater improvements in mitochondrial respiration. This raises the intriguing hypothesis that pH may affect the exercise-induced transcription of genes that regulate mitochondrial biogenesis. Eight males per...

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Bibliographic Details
Published in:PloS one 2015-12, Vol.10 (12), p.e0141317-e0141317
Main Authors: Edge, Johann, Mündel, Toby, Pilegaard, Henriette, Hawke, Emma, Leikis, Murray, Lopez-Villalobos, Nicolas, Oliveira, Rodrigo S F, Bishop, David J
Format: Article
Language:English
Subjects:
Acidosis
Acids
Adult
Ammonium
Ammonium chloride
Ammonium Chloride - pharmacology
Biosynthesis
Calcium
Calcium carbonate
Calcium Carbonate - pharmacology
Calcium chloride
Chloride
Citrate (si)-Synthase - genetics
Citrate (si)-Synthase - metabolism
Citrate synthase
Cross-Over Studies
Cytochrome
Cytochromes c - genetics
Cytochromes c - metabolism
Dehydrogenases
Double-Blind Method
Electron transport
Exercise
Fitness training programs
Forkhead Box Protein O1
Forkhead Transcription Factors - genetics
Forkhead Transcription Factors - metabolism
FOXO1 protein
Gene expression
Genetic aspects
Genetic engineering
Glucose
Glucose Transporter Type 4 - metabolism
Humans
Hydrogen-Ion Concentration
Hypotheses
Ingestion
Kinases
Male
Males
Messenger RNA
Metabolic acidosis
Metabolism
Mitochondria
Mitochondria - metabolism
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Muscles
Musculoskeletal system
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
pH effects
Physical fitness
Physiological aspects
Physiology
Proteins
Real-Time Polymerase Chain Reaction
Respiration
RNA, Messenger - metabolism
Rodents
Skeletal muscle
Transcription
Transcription Factors - genetics
Transcription Factors - metabolism
Up-Regulation - drug effects
Young Adult
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Summary:Minimizing the decrease in intracellular pH during high-intensity exercise training promotes greater improvements in mitochondrial respiration. This raises the intriguing hypothesis that pH may affect the exercise-induced transcription of genes that regulate mitochondrial biogenesis. Eight males performed 10x2-min cycle intervals at 80% VO2speak intensity on two occasions separated by ~2 weeks. Participants ingested either ammonium chloride (ACID) or calcium carbonate (PLA) the day before and on the day of the exercise trial in a randomized, counterbalanced order, using a crossover design. Biopsies were taken from the vastus lateralis muscle before and after exercise. The mRNA level of peroxisome proliferator-activated receptor co-activator 1α (PGC-1α), citrate synthase, cytochome c and FOXO1 was elevated at rest following ACID (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0141317