Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells

• Published in: PloS one 2015-12, Vol.10 (12), p.e0145068-e0145068
• Main Authors: Somaiah, Chinnapaka, Kumar, Atul, Mawrie, Darilang, Sharma, Amit, Patil, Suraj Dasharath, Bhattacharyya, Jina, Swaminathan, Rajaram, Jaganathan, Bithiah Grace
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Adhesion; Adipocytes; Adipogenesis - drug effects; Biocompatibility; Bioengineering; Biology; Biomedical materials; Bone marrow; Bone Marrow Cells - cytology; Cell adhesion; Cell adhesion & migration; Cell Adhesion - drug effects; Cell culture; Cell Differentiation - drug effects; Cell migration; Cell proliferation; Cell Proliferation - drug effects; Cell surface; Cell survival; Cell Survival - drug effects; Cells, Cultured; Collagen; Collagen - chemistry; Collagen - pharmacology; Differentiation (biology); Extracellular matrix; Extracellular Matrix Proteins - metabolism; Hematology; Homology; Humans; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Microscopy, Fluorescence; Mitochondria - metabolism; Osteogenesis - drug effects; Physiological aspects; Proteins; rhoA GTP-Binding Protein - genetics; rhoA GTP-Binding Protein - metabolism; RhoA protein; RNA, Messenger - metabolism; Spinal cord injuries; Stem cell research; Stem cells; Studies; Survival; Therapy; Tissue engineering
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0145068

Mesenchymal stem cells (MSC) can differentiate into several cell types and are desirable candidates for cell therapy and tissue engineering. However, due to poor cell survival, proliferation and differentiation in the patient, the therapy outcomes have not been satisfactory. Although several studies have been done to understand the conditions that promote proliferation, differentiation and migration of MSC in vitro and in vivo, still there is no clear understanding on the effect of non-cellular bio molecules. Of the many factors that influence the cell behavior, the immediate cell microenvironment plays a major role. In this context, we studied the effect of extracellular matrix (ECM) proteins in controlling cell survival, proliferation, migration and directed MSC differentiation. We found that collagen promoted cell proliferation, cell survival under stress and promoted high cell adhesion to the cell culture surface. Increased osteogenic differentiation accompanied by high active RHOA (Ras homology gene family member A) levels was exhibited by MSC cultured on collagen. In conclusion, our study shows that collagen will be a suitable matrix for large scale production of MSC with high survival rate and to obtain high osteogenic differentiation for therapy.