Diverse Exopolysaccharide Producing Bacteria Isolated from Milled Sugarcane: Implications for Cane Spoilage and Sucrose Yield

Bacterial deterioration of sugarcane during harvesting and processing is correlated with significant loss of sucrose yield and the accumulation of bacterial polysaccharides. Dextran, a homoglucan produced by Leuconostoc mesenteroides, has been cited as the primary polysaccharide associated with suga...

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Bibliographic Details
Published in:PloS one 2015-12, Vol.10 (12), p.e0145487-e0145487
Main Authors: Hector, Stanton, Willard, Kyle, Bauer, Rolene, Mulako, Inonge, Slabbert, Etienne, Kossmann, Jens, George, Gavin M
Format: Article
Language:English
Subjects:
Analysis
Bacteria
Bacteria - classification
Bacteria - isolation & purification
Bacteria - metabolism
Biodegradation
Biodiversity
Biotechnology
Dextran
Dextranase
Dextranase - metabolism
Enzymes
Food
Fructose
Gas chromatography
Gene amplification
Gene sequencing
Glucose
Harvesting
Leuconostoc mesenteroides
Mannose
Mechanical Phenomena
Microbial polysaccharides
Microorganisms
Monosaccharides - analysis
Oligosaccharides
Phylogenetics
Physiological aspects
Polymers
Polysaccharides
Polysaccharides, Bacterial - biosynthesis
Ribonucleic acid
Ribosomal DNA
RNA
RNA sequencing
rRNA
Saccharides
Saccharum - metabolism
Saccharum - microbiology
Saccharum officinarum
Spoilage
Sucrose
Sucrose - chemistry
Sucrose - metabolism
Sugar
Sugarcane
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Summary:Bacterial deterioration of sugarcane during harvesting and processing is correlated with significant loss of sucrose yield and the accumulation of bacterial polysaccharides. Dextran, a homoglucan produced by Leuconostoc mesenteroides, has been cited as the primary polysaccharide associated with sugarcane deterioration. A culture-based approach was used to isolate extracellular polysaccharide (EPS) producing bacterial strains from milled sugarcane stalks. Ribosomal RNA sequencing analysis grouped 25 isolates into 4 genera. This study identified 2 bacterial genera not previously associated with EPS production or sucrose degradation. All isolates produced polysaccharide when grown in the presence of sucrose. Monosaccharide analysis of purified polymers by Gas Chromatography revealed 17 EPSs consisting solely of glucose (homoglucans), while the remainder contained traces of mannose or fructose. Dextranase treatment of polysaccharides yielded full digestion profiles for only 11 extracts. Incomplete hydrolysis profiles of the remaining polysaccharides suggest the release of longer oligosaccharides which may interfere with sucrose crystal formation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0145487