The Sphingosine-1-Phosphate Lyase (LegS2) Contributes to the Restriction of Legionella pneumophila in Murine Macrophages

• Published in: PloS one 2016-01, Vol.11 (1), p.e0146410-e0146410
• Main Authors: Abu Khweek, Arwa, Kanneganti, Apurva, Guttridge D, Denis C, Amer, Amal O
• Format: Article
• Language: English
• Subjects: Activation; Aldehyde-Lyases - genetics; Aldehyde-Lyases - immunology; Analysis; Animals; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Bacterial infections; Bacterial Secretion Systems - genetics; Bacterial Secretion Systems - metabolism; Biology; Bone marrow; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Caspase; Caspase 1 - genetics; Caspase 1 - metabolism; Caspase 3 - genetics; Caspase 3 - metabolism; Caspase 7 - genetics; Caspase 7 - metabolism; Caspase-1; Caspase-3; Caspase-7; Cell adhesion & migration; Cytokines; Disease Resistance - genetics; Elongation; Enzymes; Flagellin; Fuses; Gene Expression Regulation; Homology; Host-Pathogen Interactions; Humans; Infections; Inflammation; Kinases; Laboratory animals; Legionella; Legionella pneumophila - pathogenicity; Legionella pneumophila - physiology; Legionnaires disease; Legionnaires' Disease - genetics; Legionnaires' Disease - immunology; Legionnaires' Disease - metabolism; Legionnaires' Disease - microbiology; Legionnaires' disease bacterium; Lipid metabolism; Lysophospholipids - immunology; Lysophospholipids - metabolism; Lysosomes - immunology; Lysosomes - metabolism; Lysosomes - microbiology; Macrophages; Macrophages - immunology; Macrophages - metabolism; Macrophages - microbiology; Membrane Fusion; Metabolism; Mice; Mice, Inbred NOD; Mitochondria; Mitochondrial DNA; Monocytes; Mutants; NF-kappa B - genetics; NF-kappa B - metabolism; NF-κB protein; Pathogens; Pattern recognition systems; Phagosomes - immunology; Phagosomes - metabolism; Phagosomes - microbiology; Phosphates; Phosphorylation; Physiological aspects; Pneumonia; Proteins; Replication; Signal Transduction; Species Specificity; Sphingosine; Sphingosine - analogs & derivatives; Sphingosine - immunology; Sphingosine - metabolism; Sphingosine 1-phosphate; Sphingosine 1-phosphate lyase; Sphingosine lyase; Strains (organisms); Tumor necrosis factor-TNF
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0146410

L. pneumophila is the causative agent of Legionnaires' disease, a human illness characterized by severe pneumonia. In contrast to those derived from humans, macrophages derived from most mouse strains restrict L. pneumophila replication. The restriction of L. pneumophila replication has been shown to require bacterial flagellin, a component of the type IV secretion system as well as the cytosolic NOD-like receptor (NLR) Nlrc4/ Ipaf. These events lead to caspase-1 activation which, in turn, activates caspase-7. Following caspase-7 activation, the phagosome-containing L. pneumophila fuses with the lysosome, resulting in the restriction of L. pneumophila growth. The LegS2 effector is injected by the type IV secretion system and functions as a sphingosine 1-phosphate lyase. It is homologous to the eukaryotic sphingosine lyase (SPL), an enzyme required in the terminal steps of sphingolipid metabolism. Herein, we show that mice Bone Marrow-Derived Macrophages (BMDMs) and human Monocyte-Derived Macrophages (hMDMs) are more permissive to L. pneumophila legS2 mutants than wild-type (WT) strains. This permissiveness to L. pneumophila legS2 is neither attributed to abolished caspase-1, caspase-7 or caspase-3 activation, nor due to the impairment of phagosome-lysosome fusion. Instead, an infection with the legS2 mutant resulted in the reduction of some inflammatory cytokines and their corresponding mRNA; this effect is mediated by the inhibition of the nuclear transcription factor kappa-B (NF-κB). Moreover, BMDMs infected with L. pneumophila legS2 mutant showed elongated mitochondria that resembles mitochondrial fusion. Therefore, the absence of LegS2 effector is associated with reduced NF-κB activation and atypical morphology of mitochondria.