SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis

Previous studies have demonstrated that Smyd1 plays a critical role in cardiomyocyte differentiation, cardiac morphogenesis and myofibril organization. In this study, we uncovered a novel function of Smyd1 in the regulation of endothelial cells (ECs). Our data showed that Smyd1 is expressed in vascu...

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Published in:PloS one 2016-01, Vol.11 (1), p.e0146468-e0146468
Main Authors: Ye, Xiangli, Qian, Yu, Wang, Qian, Yuan, Wuzhou, Mo, Xiaoyang, Li, Yongqing, Jiang, Zhigang, Xu, Wei, Deng, Yun, Wan, Yongqi, Fan, Xiongwei, Wu, Xiushan, Wang, Yuequn
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Binding
Biology
Biology and life sciences
Cardiomyocytes
Cell Differentiation - genetics
Cell Line
Cell migration
Cell Movement - genetics
Cell Proliferation - genetics
Deoxyribonucleic acid
DNA
DNA binding
DNA methylation
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Endothelial cells
Gene expression
Gene Expression Regulation, Developmental
Genetic aspects
Heart
Heart diseases
HEK293 Cells
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Laboratories
Life sciences
Medicine and Health Sciences
Mice
Morphogenesis
Morphogenesis - genetics
Muscle Development - genetics
Muscle Proteins - genetics
Muscle Proteins - metabolism
Neovascularization
Neovascularization, Physiologic - physiology
Protein binding
Proteins
Research and analysis methods
RNA Interference
RNA, Small Interfering - genetics
Serum response factor
Serum Response Factor - metabolism
Smooth muscle
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Previous studies have demonstrated that Smyd1 plays a critical role in cardiomyocyte differentiation, cardiac morphogenesis and myofibril organization. In this study, we uncovered a novel function of Smyd1 in the regulation of endothelial cells (ECs). Our data showed that Smyd1 is expressed in vascular endothelial cells, and knockdown of SMYD1 in endothelial cells impairs EC migration and tube formation. Furthermore, Co-IP and GST pull-down assays demonstrated that SMYD1 is associated with the Serum Response Factor (SRF). EMSA assays further showed that SMYD1 forms a complex with SRF and enhances SRF DNA binding activity. Our studies indicate that SMYD1 serves as an SRF-interacting protein, enhances SRF DNA binding activity, and is required for EC migration and tube formation to regulate angiogenesis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0146468