Single Nucleotide Polymorphisms in IL17A and IL6 Are Associated with Decreased Risk for Pulmonary Tuberculosis in Southern Brazilian Population

• Published in: PloS one 2016-02, Vol.11 (2), p.e0147814
• Main Authors: Milano, Mariana, Moraes, Milton Ozório, Rodenbusch, Rodrigo, Carvalho, Caroline Xavier, Delcroix, Melaine, Mousquer, Gabriel, Laux da Costa, Lucas, Unis, Gisela, Dalla Costa, Elis Regina, Rossetti, Maria Lucia Rosa
• Format: Article
• Language: English
• Subjects: Adult; Asthma; Biology and Life Sciences; Brazil - epidemiology; Care and treatment; Case-Control Studies; Complications and side effects; Cytokines; Development and progression; Diabetes; Disease; Female; Gene expression; Gene Frequency - genetics; Genetic aspects; Genetic Predisposition to Disease; Genotyping; Health aspects; Humans; Immune system; Infections; Interleukin 1; Interleukin 10; Interleukin 17; Interleukin 2; Interleukin 4; Interleukin 6; Interleukin-17 - genetics; Interleukin-17 - immunology; Interleukin-6 - genetics; Interleukin-6 - immunology; Male; Medicine and Health Sciences; Meta-analysis; Multiplexing; Mycobacterium tuberculosis; Mycobacterium tuberculosis - immunology; Polymorphism, Single Nucleotide; Research and Analysis Methods; Risk; Rodents; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Studies; Tuberculosis; Tuberculosis, Pulmonary - epidemiology; Tuberculosis, Pulmonary - immunology; Tuberculosis, Pulmonary - microbiology; Tumor necrosis factor; Tumor necrosis factor-TNF
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0147814

In Mycobacterium tuberculosis (MTB) infection, the complex interaction of host immune system and the mycobacteria is associated with levels of cytokines production that play a major role in determining the outcome of the disease. Several single-nucleotide polymorphisms (SNPs) in cytokine genes have been associated with tuberculosis (TB) outcome. The aim of this study was to evaluate the association between previously reported SNPs IL2-330 T>G (rs2069762); IL4-590 C>T (rs2243250); IL6-174 G>C (rs1800795); IL10-592 A>C (rs1800872); IL10-1082 G>A (rs1800896); IL17A -692 C>T (rs8193036); IL17A -197 G>A (rs2275913); TNF -238 G>A (rs361525); TNF -308 G>A (rs1800629) and IFNG +874 T>A (rs2430561) and pulmonary TB (PTB) susceptibility. We conducted a case-control study in individuals from Southern Brazil who were recruited between February 2012 and October 2013 in a high incidence TB city. We performed a multiplex genotyping assay in 191 patients with PTB and 175 healthy subjects. Our results suggest a decreased risk for PTB development associated with the IL17A -197A allele (OR = 0.29; p = 0.04), AA genotype (OR = 0.12; p = 0.04) and A carrier (AG/AA) (OR = 0.29; p = 0.004) and IL6 -174C carrier (CC/CG) (OR = 0.46; p = 0.04). We could not properly analyze IL17A -692 C>T (rs8193036) and IFNG +874T>A due to genotypic inconsistencies and found no evidence of association for the IL2, IL4, IL10 and TNF polymorphisms and PTB. In conclusion, our results show a protective effect of IL17 and IL6 polymorphisms on PTB outcome in Southern Brazilian population.