The Association of Mycobacterium avium subsp. paratuberculosis with Inflammatory Bowel Disease

The association of Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) with Crohn's disease is a controversial issue. M. paratuberculosis is detected by amplifying the IS900 gene, as microbial culture is unreliable from humans. We determined the presence of M. paratuberculosis...

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Published in:PloS one 2016-02, Vol.11 (2), p.e0148731-e0148731
Main Authors: Timms, Verlaine J, Daskalopoulos, George, Mitchell, Hazel M, Neilan, Brett A
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Alcoholic beverages
Alcohols
Animals
Antibiotics
Assaying
Biology and Life Sciences
Biopsy
Biotechnology
Cattle
Causes of
Colitis, Ulcerative - genetics
Colitis, Ulcerative - microbiology
Colonoscopy
Crohn Disease - genetics
Crohn Disease - microbiology
Crohn's disease
Crohns disease
Development and progression
Disease control
Drug Resistance, Bacterial
Endoscopy
Ethambutol
Female
Gastroenterology
Gender
Genomics
Human performance
Humans
Inflammatory bowel disease
Inflammatory bowel diseases
Intestine
IS900 gene
Male
Medicine and Health Sciences
Microorganisms
Middle Aged
Mycobacterium
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis - genetics
Mycobacterium avium subsp. paratuberculosis - growth & development
Mycobacterium avium subsp. paratuberculosis - isolation & purification
Paratuberculosis
Paratuberculosis - genetics
Paratuberculosis - microbiology
Pasteurization
Patients
Physiological aspects
Research and Analysis Methods
Rifampin
Smoking
Streptomycin
Tissue culture
Ulcerative colitis
Ulcers
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creator Timms, Verlaine J
Daskalopoulos, George
Mitchell, Hazel M
Neilan, Brett A
description The association of Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) with Crohn's disease is a controversial issue. M. paratuberculosis is detected by amplifying the IS900 gene, as microbial culture is unreliable from humans. We determined the presence of M. paratuberculosis in patients with Crohn's disease (CD) (n = 22), ulcerative colitis (UC) (n = 20), aphthous ulcers (n = 21) and controls (n = 42) using PCR assays validated on bovine tissue. Culture from human tissue was also performed. M. paratuberculosis prevalence in the CD and UC groups was compared to the prevalence in age and sex matched non-inflammatory bowel disease controls. Patients and controls were determined to be M. paratuberculosis positive if all three PCR assays were positive. A significant association was found between M. paratuberculosis and Crohn's disease (p = 0.02) that was not related to age, gender, place of birth, smoking or alcohol intake. No significant association was detected between M. paratuberculosis and UC or aphthous ulcers; however, one M. paratuberculosis isolate was successfully cultured from a patient with UC. We report the resistance of this isolate to ethambutol, rifampin, clofazamine and streptomycin. Interestingly this isolate could not only survive but could grow slowly at 5°C. We demonstrate a significant association between M. paratuberculosis and CD using multiple pre-validated PCR assays and that M. paratuberculosis can be isolated from patients with UC.
doi_str_mv 10.1371/journal.pone.0148731
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M. paratuberculosis is detected by amplifying the IS900 gene, as microbial culture is unreliable from humans. We determined the presence of M. paratuberculosis in patients with Crohn's disease (CD) (n = 22), ulcerative colitis (UC) (n = 20), aphthous ulcers (n = 21) and controls (n = 42) using PCR assays validated on bovine tissue. Culture from human tissue was also performed. M. paratuberculosis prevalence in the CD and UC groups was compared to the prevalence in age and sex matched non-inflammatory bowel disease controls. Patients and controls were determined to be M. paratuberculosis positive if all three PCR assays were positive. A significant association was found between M. paratuberculosis and Crohn's disease (p = 0.02) that was not related to age, gender, place of birth, smoking or alcohol intake. No significant association was detected between M. paratuberculosis and UC or aphthous ulcers; however, one M. paratuberculosis isolate was successfully cultured from a patient with UC. We report the resistance of this isolate to ethambutol, rifampin, clofazamine and streptomycin. Interestingly this isolate could not only survive but could grow slowly at 5°C. We demonstrate a significant association between M. paratuberculosis and CD using multiple pre-validated PCR assays and that M. paratuberculosis can be isolated from patients with UC.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26849125</pmid><doi>10.1371/journal.pone.0148731</doi><oa>free_for_read</oa></addata></record>
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subjects Adolescent
Adult
Aged
Aged, 80 and over
Alcoholic beverages
Alcohols
Animals
Antibiotics
Assaying
Biology and Life Sciences
Biopsy
Biotechnology
Cattle
Causes of
Colitis, Ulcerative - genetics
Colitis, Ulcerative - microbiology
Colonoscopy
Crohn Disease - genetics
Crohn Disease - microbiology
Crohn's disease
Crohns disease
Development and progression
Disease control
Drug Resistance, Bacterial
Endoscopy
Ethambutol
Female
Gastroenterology
Gender
Genomics
Human performance
Humans
Inflammatory bowel disease
Inflammatory bowel diseases
Intestine
IS900 gene
Male
Medicine and Health Sciences
Microorganisms
Middle Aged
Mycobacterium
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis - genetics
Mycobacterium avium subsp. paratuberculosis - growth & development
Mycobacterium avium subsp. paratuberculosis - isolation & purification
Paratuberculosis
Paratuberculosis - genetics
Paratuberculosis - microbiology
Pasteurization
Patients
Physiological aspects
Research and Analysis Methods
Rifampin
Smoking
Streptomycin
Tissue culture
Ulcerative colitis
Ulcers
title The Association of Mycobacterium avium subsp. paratuberculosis with Inflammatory Bowel Disease
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