The Impact of Ischemia/Reperfusion Injury on Liver Allografts from Deceased after Cardiac Death versus Deceased after Brain Death Donors

The shortage of organs for transplantation has led to increased use of organs procured from donors after cardiac death (DCD). The effects of cardiac death on the liver remain poorly understood, however. Using livers obtained from DCD versus donors after brain death (DBD), we aimed to understand how...

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Bibliographic Details
Published in:PloS one 2016-02, Vol.11 (2), p.e0148815-e0148815
Main Authors: Xu, Jin, Sayed, Blayne Amir, Casas-Ferreira, Ana Maria, Srinivasan, Parthi, Heaton, Nigel, Rela, Mohammed, Ma, Yun, Fuggle, Susan, Legido-Quigley, Cristina, Jassem, Wayel
Format: Article
Language:English
Subjects:
Adult
Aged
Allografts
Apoptosis
Aspartate aminotransferase
Bilirubin
Bioindicators
Biology and Life Sciences
Biopsy
Bone grafts
Brain
Brain Death
Cell death
Ceramide
Cold storage
Consent
Creatinine
Demographic aspects
Donors
Female
Grafting
Grafts
Heart diseases
Hepatology
Humans
Infiltration
Inflammation
Inflammatory response
Injuries
Ischemia
Kidneys
Life sciences
Liver
Liver - blood supply
Liver - pathology
Liver Diseases - surgery
Liver Transplantation
Liver transplants
Male
Markers
Medicine and Health Sciences
Methods
Middle Aged
Mortality
Multivariate Analysis
Organs
Patient outcomes
Patients
Pharmaceutical sciences
Reperfusion
Reperfusion Injury
Risk factors
Rodents
Studies
Surgery
Tissue Donors
Transplantation
Transplants & implants
Treatment Outcome
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Summary:The shortage of organs for transplantation has led to increased use of organs procured from donors after cardiac death (DCD). The effects of cardiac death on the liver remain poorly understood, however. Using livers obtained from DCD versus donors after brain death (DBD), we aimed to understand how ischemia/reperfusion (I/R) injury alters expression of pro-inflammatory markers ceramides and influences graft leukocyte infiltration. Hepatocyte inflammation, as assessed by ceramide expression, was evaluated in DCD (n = 13) and DBD (n = 10) livers. Allograft expression of inflammatory and cell death markers, and allograft leukocyte infiltration were evaluated from a contemporaneous independent cohort of DCD (n = 22) and DBD (n = 13) livers. When examining the differences between transplant stages in each group, C18, C20, C24 ceramides showed significant difference in DBD (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0148815