Responses in Micro-Mineral Metabolism in Rainbow Trout to Change in Dietary Ingredient Composition and Inclusion of a Micro-Mineral Premix

• Published in: PloS one 2016-02, Vol.11 (2), p.e0149378-e0149378
• Main Authors: Antony Jesu Prabhu, P, Geurden, Inge, Fontagné-Dicharry, Stéphanie, Veron, Vincent, Larroquet, Laurence, Mariojouls, Catherine, Schrama, Johan W, Kaushik, Sadasivam J
• Format: Article
• Language: English
• Subjects: Agricultural sciences; Animal biology; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animal production studies; Animals; Aquaculture; Aquaculture and Fisheries; Aquacultuur en Visserij; Bernard, Claude (1813-1878); Bioavailability; Biology and Life Sciences; Copper; Diet; Dietary minerals; Environmental science; Factorial design; Feeds; Fish; Fisheries; Fishing; Gene Expression; Genes; Genetic aspects; Glutathione; Glutathione peroxidase; Hepcidin; Ingredients; Intestine; Iron; Iron - metabolism; Leerstoelgroep Aquacultuur en Visserij; Life Sciences; Liver - metabolism; Manganese; Medicine and Health Sciences; Metabolism; Metabolites; Mineral metabolism; Minerals; Minerals - metabolism; Nutrition research; Oncorhynchus mykiss; Oncorhynchus mykiss - physiology; Peptide Elongation Factors - genetics; Peroxidase; Physiological aspects; Physiology; Proteins; Rainbow trout; Reductase; Salmo salar; Salmon; Selenium; Transcription; Transcription, Genetic; Trout; Vertebrate Zoology; WIAS; Zinc
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0149378

Responses in micro-mineral metabolism to changes in dietary ingredient composition and inclusion of a micro-mineral premix (Fe, Cu, Mn, Zn and Se) were studied in rainbow trout. In a 2 x 2 factorial design, triplicate groups of rainbow trout (initial weight: 20 g) were fed over 12 weeks at 17°C a fishmeal-based diet (M) or a plant-ingredient based diet (V), with or without inclusion of a mineral premix. Trout fed the V vs. M diet had lower feed intake, growth, hepato-somatic index, apparent availability coefficient (AAC) of Fe, Cu, Mn and Zn and also lower whole body Se and Zn concentration, whereas whole body Fe and Cu and plasma Fe concentrations were higher. Feeding the V diet increased intestinal ferric reductase activity; at transcriptional level, hepatic hepcidin expression was down-regulated and ferroportin 1 was up-regulated. Transcription of intestinal Cu-transporting ATPases and hepatic copper transporter1 were higher in V0 compared to other groups. Among the hepatic metalo-enzyme activities assayed, only Se-dependent glutathione peroxidase was affected, being lower in V fed fish. Premix inclusion reduced the AAC of Fe, Cu and Zn; increased the whole body concentration of all micro- minerals; up-regulated hepatic hepcidin and down-regulated intestinal ferroportin 1 transcription; and reduced the transcription of Cu-transporting ATPases in the intestine. Overall, the regulation of micro-mineral metabolism in rainbow trout, especially Fe and Cu, was affected both by a change in ingredient composition and micro-mineral premix inclusion.