Two Small Molecules Block Oral Epithelial Cell Invasion by Porphyromons gingivalis

Porphyromonas gingivalis is a keystone pathogen of periodontitis. One of its bacterial characteristics is the ability to invade various host cells, including nonphagocytic epithelial cells and fibroblasts, which is known to facilitate P. gingivalis adaptation and survival in the gingival environment...

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Bibliographic Details
Published in:PloS one 2016-02, Vol.11 (2), p.e0149618-e0149618
Main Authors: Ho, Meng-Hsuan, Huang, Li, Goodwin, J Shawn, Dong, Xinhong, Chen, Chin-Ho, Xie, Hua
Format: Article
Language:English
Subjects:
Actin
Bacteria
Bacterial infections
Biology and Life Sciences
Causes of
Cells, Cultured
Chinese medicine
Clathrin
Confocal microscopy
Dentistry
Development and progression
Dynamin
Dynamins - antagonists & inhibitors
Endocytosis - drug effects
Epithelial cells
Fibroblasts
Genetic aspects
Gram-negative bacteria
Humans
Hydrazones - pharmacology
Infections
Influenza
Keratinocytes
Keratinocytes - drug effects
Keratinocytes - microbiology
Ligands
Lipids
Machinery
Machinery and equipment
Medicine and Health Sciences
Membrane vesicles
Microscopy
Microtubules - microbiology
Mouth Mucosa - microbiology
Pathogens
Periodontitis
Physiological aspects
Piperidines - pharmacology
Porphyromonas gingivalis
Porphyromonas gingivalis - drug effects
Porphyromonas gingivalis - physiology
Proteins
Research and Analysis Methods
Virulence (Microbiology)
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Summary:Porphyromonas gingivalis is a keystone pathogen of periodontitis. One of its bacterial characteristics is the ability to invade various host cells, including nonphagocytic epithelial cells and fibroblasts, which is known to facilitate P. gingivalis adaptation and survival in the gingival environment. In this study, we investigated two small compounds, Alop1 and dynasore, for their role in inhibition of P. gingivalis invasion. Using confocal microscopy, we showed that these two compounds significantly reduced invasion of P. gingivalis and its outer membrane vesicles into human oral keratinocytes in a dose-dependent manner. The inhibitory effects of dynasore, a dynamin inhibitor, on the bacterial entry is consistent with the notion that P. gingivalis invasion is mediated by a clathrin-mediated endocytic machinery. We also observed that microtubule arrangement, but not actin, was altered in the host cells treated with Alop1 or dynasore, suggesting an involvement of microtubule in this inhibitory activity. This work provides an opportunity to develop compounds against P. gingivalis infection.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0149618