Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster

Lamin family proteins are structural components of a filamentous framework, the nuclear lamina (NL), underlying the inner membrane of nuclear envelope. The NL not only plays a role in nucleus mechanical support and nuclear shaping, but is also involved in many cellular processes including DNA replic...

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Bibliographic Details
Published in:PloS one 2016-03, Vol.11 (3), p.e0151231-e0151231
Main Authors: Fabbretti, Fabiana, Iannetti, Ilaria, Guglielmi, Loredana, Perconti, Susanna, Evangelistella, Chiara, Proietti De Santis, Luca, Bongiorni, Silvia, Prantera, Giorgio
Format: Article
Language:English
Subjects:
Animals
Biology
Biology and Life Sciences
Cell division
Cell Nucleus - metabolism
Chromatin
Confocal microscopy
Cytoskeletal proteins
Deoxyribonucleic acid
Differentiation
DNA
DNA biosynthesis
Drosophila
Drosophila - genetics
Drosophila melanogaster
Gene expression
Genetic aspects
Genetic engineering
Insects
Lamins - metabolism
Localization
Male
Mammals
Medicine and Health Sciences
Meiosis
Meiosis - physiology
Microscopy
Microscopy, Confocal - methods
Nuclear Envelope - metabolism
Nuclear Lamina - metabolism
Nuclei
Properties
Proteins
Research and Analysis Methods
Sperm
Spermatogenesis
Spermatogenesis - physiology
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Summary:Lamin family proteins are structural components of a filamentous framework, the nuclear lamina (NL), underlying the inner membrane of nuclear envelope. The NL not only plays a role in nucleus mechanical support and nuclear shaping, but is also involved in many cellular processes including DNA replication, gene expression and chromatin positioning. Spermatogenesis is a very complex differentiation process in which each stage is characterized by nuclear architecture dramatic changes, from the early mitotic stage to the sperm differentiation final stage. Nevertheless, very few data are present in the literature on the NL behavior during this process. Here we show the first and complete description of NL behavior during meiosis and spermatogenesis in Drosophila melanogaster. By confocal imaging, we characterized the NL modifications from mitotic stages, through meiotic divisions to sperm differentiation with an anti-laminDm0 antibody against the major component of the Drosophila NL. We observed that continuous changes in the NL structure occurred in parallel with chromatin reorganization throughout the whole process and that meiotic divisions occurred in a closed context. Finally, we analyzed NL in solofuso meiotic mutant, where chromatin segregation is severely affected, and found the strict correlation between the presence of chromatin and that of NL.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0151231