Systematic Analysis of Absorbed Anti-Inflammatory Constituents and Metabolites of Sarcandra glabra in Rat Plasma Using Ultra-High-Pressure Liquid Chromatography Coupled with Linear Trap Quadrupole Orbitrap Mass Spectrometry

• Published in: PloS one 2016-03, Vol.11 (3), p.e0150063-e0150063
• Main Authors: Li, Xiong, Zhao, Jin, Liu, Jianxing, Li, Geng, Zhao, Ya, Zeng, Xing
• Format: Article
• Language: English
• Subjects: Acids; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacokinetics; Anti-Inflammatory Agents - pharmacology; Biology and Life Sciences; Cell Line; Chinese medicine; Chromatography; Chromatography, High Pressure Liquid; Constituents; Gene expression; Genetic aspects; Herbal medicine; High pressure; High-performance liquid chromatography; Identification; Immunity; Inflammation; Lipopolysaccharides; Liquid chromatography; Macrophages; Magnoliopsida - chemistry; Male; Mass Spectrometry; Mass spectroscopy; Medical treatment; Medicine; Medicine and Health Sciences; Metabolism; Metabolites; Mice; Nitric oxide; NMR; Nuclear magnetic resonance; Oral administration; Pharmaceutical sciences; Physical Sciences; Physiological aspects; Plant Extracts - chemistry; Plant Extracts - pharmacokinetics; Plant Extracts - pharmacology; Plant metabolites; Pressure; Quadrupoles; Rats; Rats, Sprague-Dawley; Scientific imaging; Spectroscopy; Tumor necrosis factor-TNF
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0150063

Ultra-high-pressure liquid chromatography (UHPLC) was coupled with linear ion trap quadrupole Orbitrap mass spectrometry (LTQ-Orbitrap) and was used for the first time to systematically analyze the absorbed components and metabolites in rat plasma after oral administration of the water extract of Sarcandra glabra. This extract is a well-known Chinese herbal medicine for the treatment of inflammation and immunity related diseases. The anti-inflammatory activities of the absorbed components were evaluated by measuring nitric oxide (NO) production and proinflammatory genes expression in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. As a result, 54 components in Sarcandra glabra were detected in dosed rat plasma, and 36 of them were positively identified. Moreover, 23 metabolites were characterized and their originations were traced. Furthermore, 20 of the 24 studied components showed anti-inflammatory activities. These results provide evidence that this method efficiency detected constituents in plasma based on the anti-inflammatory mechanism of multiple components and would be a useful technique for screening multiple targets for natural medicine research.