Schistosomes Enhance Plasminogen Activation: The Role of Tegumental Enolase

• Published in: PLoS pathogens 2015-12, Vol.11 (12), p.e1005335-e1005335
• Main Authors: Figueiredo, Barbara C, Da'dara, Akram A, Oliveira, Sergio C, Skelly, Patrick J
• Format: Article
• Language: English
• Subjects: Animals; Binding sites; Biomedical research; Blood clots; Blotting, Western; Development and progression; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Enzymes; Helminth Proteins - metabolism; Immunohistochemistry; Membranes; Microbiological research; Mortality; Parasites; Parasitic diseases; Phosphopyruvate Hydratase - metabolism; Physiological aspects; Plasminogen - metabolism; Public health; Real-Time Polymerase Chain Reaction; RNA, Small Interfering; Schistosoma mansoni - enzymology; Schistosomiasis; Schistosomiasis mansoni - enzymology; Tissue plasminogen activator; Transfection; Tropical diseases; Variance analysis; Worms
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1005335

Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease of global public health importance. These relatively large parasites are able to survive prolonged periods in the human vasculature without inducing stable blood clots around them. We show here that the intravascular life stages (schistosomula and adult males and females) can all promote significant plasminogen (PLMG) activation in the presence of tissue plasminogen activator (tPA). This results in the generation of the potent fibrinolytic agent plasmin which could degrade blood clots forming around the worms in vivo. We demonstrate that S. mansoni enolase (SmEno) is a host-interactive tegumental enzyme that, in recombinant form, can bind PLMG and promote its activation. Like classical members of the enolase protein family, SmEno can catalyze the interconversion of 2-phospho-D-glycerate (2-PGA) and phosphoenolpyruvate (PEP). The enzyme has maximal activity at pH 7.5, requires Mg2+ for optimal activity and can be inhibited by NaF but not mefloquin. Suppressing expression of the SmEno gene significantly diminishes enolase mRNA levels, protein levels and surface enzyme activity but, surprisingly, does not affect the ability of the worms to promote PLMG activation. Thus, while SmEno can enhance PLMG activation, our analysis suggests that it is not the only contributor to the parasite's ability to perform this function. We show that the worms possess several other PLMG-binding proteins in addition to SmEno and these may have a greater importance in schistosome-driven PLMG activation.