PopulationProfiler: A Tool for Population Analysis and Visualization of Image-Based Cell Screening Data

Image-based screening typically produces quantitative measurements of cell appearance. Large-scale screens involving tens of thousands of images, each containing hundreds of cells described by hundreds of measurements, result in overwhelming amounts of data. Reducing per-cell measurements to the ave...

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Bibliographic Details
Published in:PloS one 2016-03, Vol.11 (3), p.e0151554-e0151554
Main Authors: Matuszewski, Damian J, Wählby, Carolina, Puigvert, Jordi Carreras, Sintorn, Ida-Maria
Format: Article
Language:English
Subjects:
Automation
Biology and Life Sciences
Cell cycle
Computer and Information Sciences
Computer programs
Computerized Image Processing
Cytometry
Databases, Factual
Datoriserad bildbehandling
Deoxyribonucleic acid
DNA
Drug dosages
Drugs
Engineering and Technology
Experiments
Flow cytometry
Flow Cytometry - methods
Genomes
Histograms
Image processing
Imports
Information technology
Laboratories
Medical screening
Medicin och hälsovetenskap
Medicine and Health Sciences
Methods
Microscopy
Morphology
Population
Population statistics
Research and Analysis Methods
Software
Software development tools
Statistical analysis
Statistical methods
User interface
Visualization (Computer)
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Description
Summary:Image-based screening typically produces quantitative measurements of cell appearance. Large-scale screens involving tens of thousands of images, each containing hundreds of cells described by hundreds of measurements, result in overwhelming amounts of data. Reducing per-cell measurements to the averages across the image(s) for each treatment leads to loss of potentially valuable information on population variability. We present PopulationProfiler-a new software tool that reduces per-cell measurements to population statistics. The software imports measurements from a simple text file, visualizes population distributions in a compact and comprehensive way, and can create gates for subpopulation classes based on control samples. We validate the tool by showing how PopulationProfiler can be used to analyze the effect of drugs that disturb the cell cycle, and compare the results to those obtained with flow cytometry.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0151554