A Rapid and Quantitative Flow Cytometry Method for the Analysis of Membrane Disruptive Antimicrobial Activity

We describe a microbial flow cytometry method that quantifies within 3 hours antimicrobial peptide (AMP) activity, termed Minimum Membrane Disruptive Concentration (MDC). Increasing peptide concentration positively correlates with the extent of bacterial membrane disruption and the calculated MDC is...

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Published in:PloS one 2016-03, Vol.11 (3), p.e0151694
Main Authors: O'Brien-Simpson, Neil M, Pantarat, Namfon, Attard, Troy J, Walsh, Katrina A, Reynolds, Eric C
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Anti-Bacterial Agents - pharmacology
Anti-infective agents
Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antimicrobial Cationic Peptides - pharmacology
Apoptosis
Assaying
Bacteria
Bacterial infections
Biology and Life Sciences
Cell Membrane - drug effects
Cell survival
Cytometry
Dental schools
Drug resistance
E coli
Escherichia coli
Escherichia coli - drug effects
Flow cytometry
Flow Cytometry - methods
Gram-negative bacteria
Gram-Negative Bacteria - drug effects
Gram-Positive Bacteria - drug effects
High-Throughput Screening Assays - methods
Influence
Lipids
Medicine and Health Sciences
Membranes
Membranes (Biology)
Methicillin-Resistant Staphylococcus aureus - drug effects
Microbial Sensitivity Tests - methods
Microorganisms
Minimum inhibitory concentration
Multidrug resistance
Nosocomial infections
Oral hygiene
Pathogens
Peptides
R&D
Research & development
Research and Analysis Methods
Staphylococcus aureus
Staphylococcus infections
Streptococcus infections
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cited_by cdi_FETCH-LOGICAL-c758t-26ee30b2ecefa3862d685a2dca6ac8becb57023447bca07dfcb3175540f25b263
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container_start_page e0151694
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creator O'Brien-Simpson, Neil M
Pantarat, Namfon
Attard, Troy J
Walsh, Katrina A
Reynolds, Eric C
description We describe a microbial flow cytometry method that quantifies within 3 hours antimicrobial peptide (AMP) activity, termed Minimum Membrane Disruptive Concentration (MDC). Increasing peptide concentration positively correlates with the extent of bacterial membrane disruption and the calculated MDC is equivalent to its MBC. The activity of AMPs representing three different membranolytic modes of action could be determined for a range of Gram positive and negative bacteria, including the ESKAPE pathogens, E. coli and MRSA. By using the MDC50 concentration of the parent AMP, the method provides high-throughput, quantitative screening of AMP analogues. A unique feature of the MDC assay is that it directly measures peptide/bacteria interactions and lysed cell numbers rather than bacteria survival as with MIC and MBC assays. With the threat of multi-drug resistant bacteria, this high-throughput MDC assay has the potential to aid in the development of novel antimicrobials that target bacteria with improved efficacy.
doi_str_mv 10.1371/journal.pone.0151694
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A unique feature of the MDC assay is that it directly measures peptide/bacteria interactions and lysed cell numbers rather than bacteria survival as with MIC and MBC assays. 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subjects Acinetobacter baumannii
Anti-Bacterial Agents - pharmacology
Anti-infective agents
Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antimicrobial Cationic Peptides - pharmacology
Apoptosis
Assaying
Bacteria
Bacterial infections
Biology and Life Sciences
Cell Membrane - drug effects
Cell survival
Cytometry
Dental schools
Drug resistance
E coli
Escherichia coli
Escherichia coli - drug effects
Flow cytometry
Flow Cytometry - methods
Gram-negative bacteria
Gram-Negative Bacteria - drug effects
Gram-Positive Bacteria - drug effects
High-Throughput Screening Assays - methods
Influence
Lipids
Medicine and Health Sciences
Membranes
Membranes (Biology)
Methicillin-Resistant Staphylococcus aureus - drug effects
Microbial Sensitivity Tests - methods
Microorganisms
Minimum inhibitory concentration
Multidrug resistance
Nosocomial infections
Oral hygiene
Pathogens
Peptides
R&D
Research & development
Research and Analysis Methods
Staphylococcus aureus
Staphylococcus infections
Streptococcus infections
title A Rapid and Quantitative Flow Cytometry Method for the Analysis of Membrane Disruptive Antimicrobial Activity
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