Characterization of a Chitosanase from Jelly Fig (Ficus awkeotsang Makino) Latex and Its Application in the Production of Water-Soluble Low Molecular Weight Chitosans

A chitosanase was purified from jelly fig latex by ammonium sulfate fractionation (50-80% saturation) and three successive column chromatography steps. The purified enzyme was almost homogeneous, as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and gel activity staining. The molecu...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2016-03, Vol.11 (3), p.e0150490-e0150490
Main Authors: Chang, Chen-Tien, Lin, Yen-Lu, Lu, Shu-Wei, Huang, Chun-Wei, Wang, Yu-Ting, Chung, Yun-Chin
Format: Article
Language:English
Subjects:
Ammonium
Ammonium salts
Ammonium sulfate
Ammonium Sulfate - chemistry
Antioxidants
Benzothiazoles - antagonists & inhibitors
Biological products
Biology and Life Sciences
Chemicals
Chitin
Chitin - chemistry
Chitinase
Chitosan
Chitosan - analogs & derivatives
Chitosan - chemical synthesis
Chitosan - chemistry
Chitosanase
Chromatography
Column chromatography
Deacetylation
Derivatives
Electrophoresis
Enzymes
Ethylene glycol
Ethylene glycols
Ficus - chemistry
Ficus awkeotsang
Food
Fractionation
Free Radical Scavengers - chemistry
Gel electrophoresis
Glycoside Hydrolases - chemistry
Glycoside Hydrolases - isolation & purification
Hydrogen-Ion Concentration
Hydrolysis
Insects
Isoelectric focusing
Isoelectric Point
Kinetics
Latex
Latex - chemistry
Low molecular weights
Microorganisms
Molecular Weight
Nutrition
Peroxides - antagonists & inhibitors
Peroxyl radicals
pH effects
Physical Sciences
Plant Proteins - chemistry
Plant Proteins - isolation & purification
Polyimide resins
Polymers
Proteins
Sodium lauryl sulfate
Solubility
Studies
Substrate Specificity
Substrates
Sulfate
Sulfates
Sulfonic Acids - antagonists & inhibitors
Superoxide
Superoxides
Superoxides - antagonists & inhibitors
Water
Water purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A chitosanase was purified from jelly fig latex by ammonium sulfate fractionation (50-80% saturation) and three successive column chromatography steps. The purified enzyme was almost homogeneous, as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and gel activity staining. The molecular mass of the enzyme was 20.5 kDa. The isoelectric point (pI) was
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0150490