KatG, the Bifunctional Catalase of Xanthomonas citri subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves

• Published in: PloS one 2016-03, Vol.11 (3), p.e0151657
• Main Authors: Tondo, María Laura, Delprato, María Laura, Kraiselburd, Ivana, Fernández Zenoff, María Verónica, Farías, María Eugenia, Orellano, Elena G
• Format: Article
• Language: English
• Subjects: Aerobic respiration; Bacteria; Biofilms; Biofilms - growth & development; Biology and Life Sciences; Canker; Catalase; Catalase - genetics; Catalase - metabolism; Citrus; Citrus sinensis - microbiology; Deficient mutant; Diseases; Exposure; Gene expression; Gene Expression Regulation, Bacterial; Genetic aspects; Genomes; Hydrogen; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Immune response; Immune system; Immunological tolerance; Intracellular levels; KatG gene; Leaves; Life assessment; Life cycle engineering; Life cycles; Molecular biology; Mutation; Oxidation; Oxidative stress; Oxidative Stress - physiology; Oxygen; Peroxides; Physical Sciences; Physiological aspects; Physiology; Phytopathogenic bacteria; Plant Diseases - microbiology; Plant Leaves - microbiology; Plasmids; Proteomics; Radiation; Reactive oxygen species; Staphylococcus aureus; Survival; Toxicity; U.V. radiation; Ultraviolet radiation; Ultraviolet Rays; Xanthomonas - enzymology; Xanthomonas - metabolism; Xanthomonas - radiation effects; Xanthomonas citri
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0151657

Xanthomonas citri subsp. citri (Xcc) is the bacterium responsible for citrus canker. This bacterium is exposed to reactive oxygen species (ROS) at different points during its life cycle, including those normally produced by aerobic respiration or upon exposition to ultraviolet (UV) radiation. Moreover, ROS are key components of the host immune response. Among enzymatic ROS-detoxifying mechanisms, catalases eliminate H2O2, avoiding the potential damage caused by this specie. Xcc genome includes four catalase genes. In this work, we studied the physiological role of KatG, the only bifunctional catalase of Xcc, through the construction and characterization of a modified strain (XcckatG), carrying an insertional mutation in the katG gene. First, we evaluated the involvement of KatG in the bacterial adaptive response to H2O2. XcckatG cultures exhibited lower catalase activity than those of the wild-type strain, and this activity was not induced upon treatment with sub-lethal doses of H2O2. Moreover, the KatG-deficient mutant exhibited decreased tolerance to H2O2 toxicity compared to wild-type cells and accumulated high intracellular levels of peroxides upon exposure to sub-lethal concentrations of H2O2. To further study the role of KatG in Xcc physiology, we evaluated bacterial survival upon exposure to UV-A or UV-B radiation. In both conditions, XcckatG showed a high mortality in comparison to Xcc wild-type. Finally, we studied the development of bacterial biofilms. While structured biofilms were observed for the Xcc wild-type, the development of these structures was impaired for XcckatG. Based on these results, we demonstrated that KatG is responsible for Xcc adaptive response to H2O2 and a key component of the bacterial response to oxidative stress. Moreover, this enzyme plays an important role during Xcc epiphytic survival, being essential for biofilm formation and UV resistance.