Regionally Discrete Aqueous Humor Outflow Quantification Using Fluorescein Canalograms

To visualize and quantify conventional outflow directly in its anatomic location. We obtained fluorescein canalograms in six porcine whole eyes and six porcine anterior segment cultures. Eyes were perfused with a constant pressure of 15 mmHg using media containing 0.017 mg/ml fluorescein. Flow patte...

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Bibliographic Details
Published in:PloS one 2016-03, Vol.11 (3), p.e0151754-e0151754
Main Authors: Loewen, Ralitsa T, Brown, Eric N, Roy, Pritha, Schuman, Joel S, Sigal, Ian A, Loewen, Nils A
Format: Article
Language:English
Subjects:
Algorithms
Analysis
Animals
Anterior chamber
Anterior Chamber - metabolism
Aqueous Humor - metabolism
Biology and Life Sciences
Blood flow
Convergence
Cornea
Eye
Eye (anatomy)
Fluorescein
Fluorescein - metabolism
Fluorescence
Fluorimetry
Fluoroscopic imaging
Generalized linear models
Glaucoma
Imaging, Three-Dimensional
Medicine
Medicine and Health Sciences
Ophthalmology - instrumentation
Optics
Outflow
Quadrants
Regional analysis
Regional development
Research and Analysis Methods
Stem cells
Sus scrofa
Time-Lapse Imaging
Tomography
Ventricular function
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Summary:To visualize and quantify conventional outflow directly in its anatomic location. We obtained fluorescein canalograms in six porcine whole eyes and six porcine anterior segment cultures. Eyes were perfused with a constant pressure of 15 mmHg using media containing 0.017 mg/ml fluorescein. Flow patterns were visualized using a stereo dissecting microscope equipped for fluorescent imaging. Images were captured every 30 seconds for 20 minutes for time lapse analysis. Anterior chamber cultures were imaged again on day three of culture. Canalograms were first analyzed for filling time per quadrant. We then wrote a program to automatically compute focal flow fits for each macropixel and to detect convergent perilimbal flow patterns with macropixels grouped into 3 equal-radial width rings around the cornea. A generalized additive model was used to determine fluorescence changes of individual macropixels. The resulting imaging algorithm deployed 1024 macropixels that were fit to determine maximum intensity and time to fill. These individual fits highlighted the focal flow function. In whole eyes, significantly faster flow was seen in the inferonasal (IN) and superonasal (SN) quadrants compared to the superotemporal (ST) and inferotemporal (IT) ones (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0151754