Association between SIRT1 Gene Polymorphisms and Breast Cancer in Egyptians

• Published in: PloS one 2016-03, Vol.11 (3), p.e0151901-e0151901
• Main Authors: Rizk, Sherine M, Shahin, Nancy N, Shaker, Olfat G
• Format: Article
• Language: English
• Subjects: Adult; Aged; Alleles; Analysis; Biochemistry; Biology and Life Sciences; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cancer; Case-Control Studies; Chemotherapy; Demography; Deoxyribonucleic acid; Disease; DNA; Egypt; Epigenetics; Estrogen receptors; Estrogens; Female; Females; Gene expression; Gene frequency; Gene Frequency - genetics; Gene polymorphism; Genetic Association Studies; Genetic polymorphisms; Genetic Predisposition to Disease; Genomes; Genomics; Genotypes; Health risks; Heredity; Homology; Humans; Laboratories; Linkage Disequilibrium - genetics; Lymph nodes; Lymphatic system; Mating; Medical prognosis; Medicine and Health Sciences; Middle Aged; Patients; Pharmacy; Physiology; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide - genetics; Progesterone; Prognosis; Prostate; Proteins; Risk factors; Single-nucleotide polymorphism; SIRT1 protein; Sirtuin 1 - blood; Sirtuin 1 - genetics; Tumorigenesis; Tumors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0151901

Breast cancer is reported to cause the highest mortality among female cancer patients. Previous studies have explored the association of silent mating-type information regulator 2 homolog 1 (SIRT1) gene expression with prognosis in breast cancer. However, no studies exist, so far, on the role of SIRT1 gene polymorphism in breast cancer risk or prognosis. The present study aimed to assess the association between SIRT1 gene polymorphisms and breast cancer in Egyptians. The study comprised 980 Egyptian females divided into a breast cancer group (541 patients) and a healthy control group (439 subjects). SIRT1 gene single nucleotide polymorphisms (SNPs) rs3758391, rs3740051 and rs12778366 were genotyped using real-time polymerase chain reaction (RT-PCR). Allelic and genotypic frequencies were determined in both groups and association with breast cancer and clinicopathological characteristics was assessed. Breast cancer patients exhibited elevated serum SIRT1 levels which varied among different tumor grades. SIRT1 rs3758391 and rs12778366 TT genotypes were more frequent, exhibited higher SIRT1 levels than CC and CT genotypes and were associated with histologic grade and lymph node status. SIRT1 rs12778366 TT genotype also correlated with negative estrogen receptor (ER) and progesterone receptor (PR) statuses. The T allele frequency for both SNPs was higher in breast cancer patients than in normal subjects. Combined GG and AG genotypes of rs3740051 were more frequent, showed higher serum SIRT1 levels than the AA genotype, and were associated with ER and PR expression. Furthermore, inheritance of the G allele was associated with breast cancer. Our findings reveal that rs3758391 and rs12778366 polymorphisms of SIRT1 gene are associated with breast cancer risk and prognosis in the Egyptian population.