Time Gating of Chloroplast Autofluorescence Allows Clearer Fluorescence Imaging In Planta

Chloroplast, an organelle facilitating photosynthesis, exhibits strong autofluorescence, which is an undesired background signal that restricts imaging experiments with exogenous fluorophore in plants. In this study, the autofluorescence was characterized in planta under confocal laser microscopy, a...

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Bibliographic Details
Published in:PloS one 2016-03, Vol.11 (3), p.e0152484-e0152484
Main Author: Kodama, Yutaka
Format: Article
Language:English
Subjects:
Agrobacterium
Arabidopsis - metabolism
Biology and Life Sciences
Carotenoids
Chloroplasts
Chloroplasts - metabolism
Confocal
Engineering and Technology
Experiments
Fluorescence
Fluorescence microscopy
Gating
Genetic aspects
Glycine max
Hepatophyta - metabolism
Imaging
Laser microscopy
Lasers
Light
Localization
Marchantia polymorpha
Microscopy
Optical Imaging - methods
Photosynthesis
Physical Sciences
Physiological aspects
Physiology
Proteins
Research and Analysis Methods
Sensors
Spectrum analysis
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Summary:Chloroplast, an organelle facilitating photosynthesis, exhibits strong autofluorescence, which is an undesired background signal that restricts imaging experiments with exogenous fluorophore in plants. In this study, the autofluorescence was characterized in planta under confocal laser microscopy, and it was found that the time-gated imaging technique completely eliminates the autofluorescence. As a demonstration of the technique, a clearer signal of fluorescent protein-tagged phototropin, a blue-light photoreceptor localized at the chloroplast periphery, was visualized in planta.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0152484