Screening of Compounds against Gardnerella vaginalis Biofilms

Bacterial vaginosis (BV) is a common infection in reproductive age woman and is characterized by dysbiosis of the healthy vaginal flora which is dominated by Lactobacilli, followed by growth of bacteria like Gardnerella vaginalis. The ability of G. vaginalis to form biofilms contributes to the high...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2016-04, Vol.11 (4), p.e0154086-e0154086
Main Authors: Gottschick, Cornelia, Szafranski, Szymon P, Kunze, Brigitte, Sztajer, Helena, Masur, Clarissa, Abels, Christoph, Wagner-Döbler, Irene
Format: Article
Language:English
Subjects:
Anaerobic bacteria
Anti-Bacterial Agents
Antibiotics
Bacteria
Biofilms
Biology and Life Sciences
Biomass
Catheters
Data processing
Diagnosis
Disease
Disintegration
Dysbacteriosis
Female
Flora
Gardnerella vaginalis
Gardnerella vaginalis - drug effects
Genital microflora
Humans
Infections
Lactobacilli
Lactobacillus
Medical screening
Medicine and Health Sciences
Metronidazole
Microbial mats
Microbial Sensitivity Tests
Models, Biological
Physical Sciences
Research and Analysis Methods
Risk factors
Sodium
Tobramycin
Vagina
Vaginosis
Vaginosis, Bacterial - drug therapy
Vaginosis, Bacterial - microbiology
Viability
Womens health
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bacterial vaginosis (BV) is a common infection in reproductive age woman and is characterized by dysbiosis of the healthy vaginal flora which is dominated by Lactobacilli, followed by growth of bacteria like Gardnerella vaginalis. The ability of G. vaginalis to form biofilms contributes to the high rates of recurrence that are typical for BV and which unfortunately make repeated antibiotic therapy inevitable. Here we developed a biofilm model for G. vaginalis and screened a large spectrum of compounds for their ability to prevent biofilm formation and to resolve an existing G. vaginalis biofilm. The antibiotics metronidazole and tobramycin were highly effective in preventing biofilm formation, but had no effect on an established biofilm. The application of the amphoteric tenside sodium cocoamphoacetate (SCAA) led to disintegration of existing biofilms, reducing biomass by 51% and viability by 61% and it was able to increase the effect of metronidazole by 40% (biomass) and 61% (viability). Our data show that attacking the biofilm and the bacterial cells by the combination of an amphoteric tenside with the antibiotic metronidazole might be a useful strategy against BV.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0154086