IL12, IL10, IFNγ and TNFα Expression in Human Primary Monocytes Stimulated with Bacterial Heat Shock GroEL (Hsp64) Protein

• Published in: PloS one 2016-04, Vol.11 (4), p.e0154085-e0154085
• Main Authors: Nalbant, Ayten, Saygılı, Tahsin
• Format: Article
• Language: English
• Subjects: Adaptive immunity; Aggregatibacter actinomycetemcomitans; Aggregatibacter actinomycetemcomitans - immunology; Antigens; Bacteria; Bacterial Proteins - immunology; Biology and Life Sciences; CD14 antigen; Cells, Cultured; Chaperonin 60 - immunology; Cytokines; Cytometry; Dendritic cells; Ethics; Flow cytometry; Granulocytes; GroEL protein; Heat shock; Heat shock proteins; Helicobacter pylori; Humans; Immune response; Immune system; Immunity, Innate; Immunology; Inflammatory diseases; Interferon-gamma - immunology; Interleukin 1; Interleukin 10; Interleukin 12; Interleukin-10 - immunology; Interleukin-12 - immunology; Kinetics; Laboratories; Leukocytes (mononuclear); Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - microbiology; Lymphocytes; Medicine and Health Sciences; Molecular biology; Monocytes; Mycobacterium tuberculosis; Oral cavity; Pasteurellaceae Infections - immunology; Periodontal diseases; Peripheral blood mononuclear cells; Statistical analysis; Tuberculosis; Tumor Necrosis Factor-alpha - immunology; Tumor necrosis factor-TNF; Tumor necrosis factor-α; γ-Interferon
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0154085

Actinobacillus (Aggregatibacter) actinomycetemicomitans (Aa) is a bacterium that lives in the oral cavity and plays an important role in periodontal diseases. The effect of A.actinomycetemcomitans's heat shock family protein GroEL on host or immune cells including monocytes is quite limited. In this study, the recombinant A.actinomycetemcomitans's GroEL protein (rAaGroEL) was used as an antigen and its effects on monocytes of peripheral blood mononuclear cells (PBMCs) was investigated. To do that, PBMCs were stimulated with rAaGroEL protein at different time points (16h to 96h) and the cytokines of CD14+ monocytes were detected with intracellular cytokine staining by Flow cytometry. Data showed that AaGroEL protein has an antigenic effect on human primary monocytes. AaGroEL protein responsive CD14 monocytes stimulates the expression of IL12, IL10, IFNγ and TNFα cytokines with different kinetics and expression profile. Therefore, A. actinomycetemcomitans's heat shock GroEL protein can modulate innate and adaptive immune responses and contribute to an inflammatory diseases pathology.