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Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice

The critical node (CN) in seed aging in rice (Oryza sativa) is the transformation from Phase I (P-I) to Phase II (P-II) of the reverse S-shaped curve (RS-SC). Although mitochondrial dysfunction plays a key role in seed ageing, the metabolic shift in the CN remains poorly understood. Here, we investi...

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Published in:PloS one 2016-04, Vol.11 (4), p.e0148013-e0148013
Main Authors: Yin, Guangkun, Whelan, James, Wu, Shuhua, Zhou, Jing, Chen, Baoyin, Chen, Xiaoling, Zhang, Jinmei, He, Juanjuan, Xin, Xia, Lu, Xinxiong
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creator Yin, Guangkun
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He, Juanjuan
Xin, Xia
Lu, Xinxiong
description The critical node (CN) in seed aging in rice (Oryza sativa) is the transformation from Phase I (P-I) to Phase II (P-II) of the reverse S-shaped curve (RS-SC). Although mitochondrial dysfunction plays a key role in seed ageing, the metabolic shift in the CN remains poorly understood. Here, we investigated the mitochondrial regulatory mechanisms during the CN of rice seed ageing. We showed that during the CN of seed ageing, the mitochondrial ultrastructure was impaired, causing oxygen consumption to decrease, along with cytochrome c (cyt c) oxidase and malate dehydrogenase (MDH) activity. In addition, the transcript levels for the alternative pathway of the electron transport chain (ETC) were significantly induced, whereas the transcripts of the cytochrome oxidase (COX) pathway were inhibited. These changes were concomitant with the down-regulation of mitochondrial protein levels related to carbon and nitrogen metabolism, ATP synthase (ATPase) complex, tricarboxylic acid cycle (TCA) cycle, mitochondrial oxidative enzymes, and a variety of other proteins. Therefore, while these responses inhibit the production of ATP and its intermediates, signals from mitochondria (such as the decrease of cyt c and accumulation of reactive oxygen species (ROS)) may also induce oxidative damage. These events provide considerable information about the mitochondrial metabolic shifts involved in the progression of seed ageing in the CN.
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Although mitochondrial dysfunction plays a key role in seed ageing, the metabolic shift in the CN remains poorly understood. Here, we investigated the mitochondrial regulatory mechanisms during the CN of rice seed ageing. We showed that during the CN of seed ageing, the mitochondrial ultrastructure was impaired, causing oxygen consumption to decrease, along with cytochrome c (cyt c) oxidase and malate dehydrogenase (MDH) activity. In addition, the transcript levels for the alternative pathway of the electron transport chain (ETC) were significantly induced, whereas the transcripts of the cytochrome oxidase (COX) pathway were inhibited. These changes were concomitant with the down-regulation of mitochondrial protein levels related to carbon and nitrogen metabolism, ATP synthase (ATPase) complex, tricarboxylic acid cycle (TCA) cycle, mitochondrial oxidative enzymes, and a variety of other proteins. Therefore, while these responses inhibit the production of ATP and its intermediates, signals from mitochondria (such as the decrease of cyt c and accumulation of reactive oxygen species (ROS)) may also induce oxidative damage. These events provide considerable information about the mitochondrial metabolic shifts involved in the progression of seed ageing in the CN.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27124767</pmid><doi>10.1371/journal.pone.0148013</doi><oa>free_for_read</oa></addata></record>
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subjects Adenosine triphosphatase
Adenosine Triphosphatases - metabolism
Adenosine triphosphate
Aging
Aging (Biology)
Aging - metabolism
Apoptosis
Arabidopsis
ATP
ATP synthase
Biology and Life Sciences
Biosynthesis
Carbon - metabolism
Citric Acid Cycle - physiology
Crop science
Cytochrome
Cytochrome c
Cytochromes c - metabolism
Damage accumulation
Down-Regulation - physiology
Electron transport
Electron transport chain
Electron Transport Complex IV - metabolism
Enzymes
Gene expression
Intermediates
Malate
Malate dehydrogenase
Malate Dehydrogenase - metabolism
Metabolism
Mitochondria
Mitochondria - metabolism
Mitochondrial Proteins - metabolism
Nitrogen - metabolism
Oryza - metabolism
Oryza sativa
Oxidase
Oxidation-Reduction
Oxidative stress
Oxidative Stress - physiology
Oxygen
Oxygen consumption
Oxygen Consumption - physiology
Pennisetum glaucum
Phase transitions
Physiological aspects
Plant metabolism
Plant mitochondria
Proteins
Reactive oxygen species
Reactive Oxygen Species - metabolism
Regulatory mechanisms (biology)
Research and Analysis Methods
Rice
Seeds
Seeds - metabolism
Studies
Transcription
Transformation
Tricarboxylic acid cycle
Ultrastructure
title Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice
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