A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids

A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydr...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2016-05, Vol.11 (5), p.e0154610-e0154610
Main Authors: Massai, Diana, Isu, Giuseppe, Madeddu, Denise, Cerino, Giulia, Falco, Angela, Frati, Caterina, Gallo, Diego, Deriu, Marco A, Falvo D'Urso Labate, Giuseppe, Quaini, Federico, Audenino, Alberto, Morbiducci, Umberto
Format: Article
Language:English
Subjects:
Aerospace engineering
Apoptosis
Biological effects
Biology and Life Sciences
Biophysical Phenomena
Bioreactors
Bones
Cancer
Cell culture
Cell Culture Techniques - instrumentation
Cell growth
Cell Line, Tumor
Computational fluid dynamics
Computer Simulation
Deoxyribonucleic acid
DNA
DNA damage
Earth Sciences
Equipment Design
Fluid flow
Humans
Hydrodynamics
Laboratories
Lung carcinoma
Mass transport
Medicine
Microscopy, Electron, Transmission
Models, Biological
Physical Sciences
Process controls
Research and Analysis Methods
Sedimentation & deposition
Shear stress
Shear stresses
Spheroids
Spheroids, Cellular - cytology
Stem cells
Tissue engineering
Tumor Cells, Cultured - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c559t-27426a8d12426d850edb30167f320dda4b2407f7343ce5406088e5d404a2b82e3
cites cdi_FETCH-LOGICAL-c559t-27426a8d12426d850edb30167f320dda4b2407f7343ce5406088e5d404a2b82e3
container_end_page e0154610
container_issue 5
container_start_page e0154610
container_title PloS one
container_volume 11
creator Massai, Diana
Isu, Giuseppe
Madeddu, Denise
Cerino, Giulia
Falco, Angela
Frati, Caterina
Gallo, Diego
Deriu, Marco A
Falvo D'Urso Labate, Giuseppe
Quaini, Federico
Audenino, Alberto
Morbiducci, Umberto
description A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future.
doi_str_mv 10.1371/journal.pone.0154610
format article
fullrecord <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1786792952</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_bcbf65bc490242aaa0dd20064d3d19b5</doaj_id><sourcerecordid>4044902261</sourcerecordid><originalsourceid>FETCH-LOGICAL-c559t-27426a8d12426d850edb30167f320dda4b2407f7343ce5406088e5d404a2b82e3</originalsourceid><addsrcrecordid>eNqFUl1rFDEUHUSx7eo_EA340pdd853Mi7AdvwoFH6q-hkxyp5tldjImM0L_vam7W1oRfAg35J5zcnJzquoVwSvCFHm3jXMabL8a4wArTASXBD-pTknN6FJSzJ4-2J9UZzlvMRZMS_m8OqGKcM6wPK3iGv2AlO0UekAXISawbooJdWV9uB3sLjh0PecRhhzigBroe9TM_TQnWKH1OPbBFW7pTBFNGzj2UOxQYwcHaU-5HjeQYvD5RfWss32Gl4e6qL5_-vit-bK8-vr5sllfLZ0Q9bSkilNptSe0VK8FBt8yTKTqGMXeW95SjlWnGGcOBMcSaw3Cc8wtbTUFtqje7HXHPmZzmFU2RGmpaloLWhCXe4SPdmvGFHY23Zpog_lzENONsWkKrgfTuraTonW8xsWPtbZYoBhL7pkndSuK1vvDbXO7A-9gmJLtH4k-7gxhY27iL8O1kEyzInB-EEjx5wx5MruQXZmcHSDOxbfGQpT_w-r_UKUVV4oV4UX19i_ovwfB9yiXYs4JunvfBJu7qB1Z5i5q5hC1Qnv98M33pGO22G8m4tEQ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1786792952</pqid></control><display><type>article</type><title>A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central</source><creator>Massai, Diana ; Isu, Giuseppe ; Madeddu, Denise ; Cerino, Giulia ; Falco, Angela ; Frati, Caterina ; Gallo, Diego ; Deriu, Marco A ; Falvo D'Urso Labate, Giuseppe ; Quaini, Federico ; Audenino, Alberto ; Morbiducci, Umberto</creator><contributor>Pesce, Maurizio</contributor><creatorcontrib>Massai, Diana ; Isu, Giuseppe ; Madeddu, Denise ; Cerino, Giulia ; Falco, Angela ; Frati, Caterina ; Gallo, Diego ; Deriu, Marco A ; Falvo D'Urso Labate, Giuseppe ; Quaini, Federico ; Audenino, Alberto ; Morbiducci, Umberto ; Pesce, Maurizio</creatorcontrib><description>A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0154610</identifier><identifier>PMID: 27144306</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aerospace engineering ; Apoptosis ; Biological effects ; Biology and Life Sciences ; Biophysical Phenomena ; Bioreactors ; Bones ; Cancer ; Cell culture ; Cell Culture Techniques - instrumentation ; Cell growth ; Cell Line, Tumor ; Computational fluid dynamics ; Computer Simulation ; Deoxyribonucleic acid ; DNA ; DNA damage ; Earth Sciences ; Equipment Design ; Fluid flow ; Humans ; Hydrodynamics ; Laboratories ; Lung carcinoma ; Mass transport ; Medicine ; Microscopy, Electron, Transmission ; Models, Biological ; Physical Sciences ; Process controls ; Research and Analysis Methods ; Sedimentation &amp; deposition ; Shear stress ; Shear stresses ; Spheroids ; Spheroids, Cellular - cytology ; Stem cells ; Tissue engineering ; Tumor Cells, Cultured - cytology</subject><ispartof>PloS one, 2016-05, Vol.11 (5), p.e0154610-e0154610</ispartof><rights>2016 Massai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Massai et al 2016 Massai et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-27426a8d12426d850edb30167f320dda4b2407f7343ce5406088e5d404a2b82e3</citedby><cites>FETCH-LOGICAL-c559t-27426a8d12426d850edb30167f320dda4b2407f7343ce5406088e5d404a2b82e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1786792952/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1786792952?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,37012,44589,53790,53792,74997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27144306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pesce, Maurizio</contributor><creatorcontrib>Massai, Diana</creatorcontrib><creatorcontrib>Isu, Giuseppe</creatorcontrib><creatorcontrib>Madeddu, Denise</creatorcontrib><creatorcontrib>Cerino, Giulia</creatorcontrib><creatorcontrib>Falco, Angela</creatorcontrib><creatorcontrib>Frati, Caterina</creatorcontrib><creatorcontrib>Gallo, Diego</creatorcontrib><creatorcontrib>Deriu, Marco A</creatorcontrib><creatorcontrib>Falvo D'Urso Labate, Giuseppe</creatorcontrib><creatorcontrib>Quaini, Federico</creatorcontrib><creatorcontrib>Audenino, Alberto</creatorcontrib><creatorcontrib>Morbiducci, Umberto</creatorcontrib><title>A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future.</description><subject>Aerospace engineering</subject><subject>Apoptosis</subject><subject>Biological effects</subject><subject>Biology and Life Sciences</subject><subject>Biophysical Phenomena</subject><subject>Bioreactors</subject><subject>Bones</subject><subject>Cancer</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Computational fluid dynamics</subject><subject>Computer Simulation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Earth Sciences</subject><subject>Equipment Design</subject><subject>Fluid flow</subject><subject>Humans</subject><subject>Hydrodynamics</subject><subject>Laboratories</subject><subject>Lung carcinoma</subject><subject>Mass transport</subject><subject>Medicine</subject><subject>Microscopy, Electron, Transmission</subject><subject>Models, Biological</subject><subject>Physical Sciences</subject><subject>Process controls</subject><subject>Research and Analysis Methods</subject><subject>Sedimentation &amp; deposition</subject><subject>Shear stress</subject><subject>Shear stresses</subject><subject>Spheroids</subject><subject>Spheroids, Cellular - cytology</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Tumor Cells, Cultured - cytology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqFUl1rFDEUHUSx7eo_EA340pdd853Mi7AdvwoFH6q-hkxyp5tldjImM0L_vam7W1oRfAg35J5zcnJzquoVwSvCFHm3jXMabL8a4wArTASXBD-pTknN6FJSzJ4-2J9UZzlvMRZMS_m8OqGKcM6wPK3iGv2AlO0UekAXISawbooJdWV9uB3sLjh0PecRhhzigBroe9TM_TQnWKH1OPbBFW7pTBFNGzj2UOxQYwcHaU-5HjeQYvD5RfWss32Gl4e6qL5_-vit-bK8-vr5sllfLZ0Q9bSkilNptSe0VK8FBt8yTKTqGMXeW95SjlWnGGcOBMcSaw3Cc8wtbTUFtqje7HXHPmZzmFU2RGmpaloLWhCXe4SPdmvGFHY23Zpog_lzENONsWkKrgfTuraTonW8xsWPtbZYoBhL7pkndSuK1vvDbXO7A-9gmJLtH4k-7gxhY27iL8O1kEyzInB-EEjx5wx5MruQXZmcHSDOxbfGQpT_w-r_UKUVV4oV4UX19i_ovwfB9yiXYs4JunvfBJu7qB1Z5i5q5hC1Qnv98M33pGO22G8m4tEQ</recordid><startdate>20160504</startdate><enddate>20160504</enddate><creator>Massai, Diana</creator><creator>Isu, Giuseppe</creator><creator>Madeddu, Denise</creator><creator>Cerino, Giulia</creator><creator>Falco, Angela</creator><creator>Frati, Caterina</creator><creator>Gallo, Diego</creator><creator>Deriu, Marco A</creator><creator>Falvo D'Urso Labate, Giuseppe</creator><creator>Quaini, Federico</creator><creator>Audenino, Alberto</creator><creator>Morbiducci, Umberto</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20160504</creationdate><title>A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids</title><author>Massai, Diana ; Isu, Giuseppe ; Madeddu, Denise ; Cerino, Giulia ; Falco, Angela ; Frati, Caterina ; Gallo, Diego ; Deriu, Marco A ; Falvo D'Urso Labate, Giuseppe ; Quaini, Federico ; Audenino, Alberto ; Morbiducci, Umberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c559t-27426a8d12426d850edb30167f320dda4b2407f7343ce5406088e5d404a2b82e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aerospace engineering</topic><topic>Apoptosis</topic><topic>Biological effects</topic><topic>Biology and Life Sciences</topic><topic>Biophysical Phenomena</topic><topic>Bioreactors</topic><topic>Bones</topic><topic>Cancer</topic><topic>Cell culture</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Computational fluid dynamics</topic><topic>Computer Simulation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>Earth Sciences</topic><topic>Equipment Design</topic><topic>Fluid flow</topic><topic>Humans</topic><topic>Hydrodynamics</topic><topic>Laboratories</topic><topic>Lung carcinoma</topic><topic>Mass transport</topic><topic>Medicine</topic><topic>Microscopy, Electron, Transmission</topic><topic>Models, Biological</topic><topic>Physical Sciences</topic><topic>Process controls</topic><topic>Research and Analysis Methods</topic><topic>Sedimentation &amp; deposition</topic><topic>Shear stress</topic><topic>Shear stresses</topic><topic>Spheroids</topic><topic>Spheroids, Cellular - cytology</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Tumor Cells, Cultured - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Massai, Diana</creatorcontrib><creatorcontrib>Isu, Giuseppe</creatorcontrib><creatorcontrib>Madeddu, Denise</creatorcontrib><creatorcontrib>Cerino, Giulia</creatorcontrib><creatorcontrib>Falco, Angela</creatorcontrib><creatorcontrib>Frati, Caterina</creatorcontrib><creatorcontrib>Gallo, Diego</creatorcontrib><creatorcontrib>Deriu, Marco A</creatorcontrib><creatorcontrib>Falvo D'Urso Labate, Giuseppe</creatorcontrib><creatorcontrib>Quaini, Federico</creatorcontrib><creatorcontrib>Audenino, Alberto</creatorcontrib><creatorcontrib>Morbiducci, Umberto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Massai, Diana</au><au>Isu, Giuseppe</au><au>Madeddu, Denise</au><au>Cerino, Giulia</au><au>Falco, Angela</au><au>Frati, Caterina</au><au>Gallo, Diego</au><au>Deriu, Marco A</au><au>Falvo D'Urso Labate, Giuseppe</au><au>Quaini, Federico</au><au>Audenino, Alberto</au><au>Morbiducci, Umberto</au><au>Pesce, Maurizio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-05-04</date><risdate>2016</risdate><volume>11</volume><issue>5</issue><spage>e0154610</spage><epage>e0154610</epage><pages>e0154610-e0154610</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27144306</pmid><doi>10.1371/journal.pone.0154610</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2016-05, Vol.11 (5), p.e0154610-e0154610
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1786792952
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central
subjects Aerospace engineering
Apoptosis
Biological effects
Biology and Life Sciences
Biophysical Phenomena
Bioreactors
Bones
Cancer
Cell culture
Cell Culture Techniques - instrumentation
Cell growth
Cell Line, Tumor
Computational fluid dynamics
Computer Simulation
Deoxyribonucleic acid
DNA
DNA damage
Earth Sciences
Equipment Design
Fluid flow
Humans
Hydrodynamics
Laboratories
Lung carcinoma
Mass transport
Medicine
Microscopy, Electron, Transmission
Models, Biological
Physical Sciences
Process controls
Research and Analysis Methods
Sedimentation & deposition
Shear stress
Shear stresses
Spheroids
Spheroids, Cellular - cytology
Stem cells
Tissue engineering
Tumor Cells, Cultured - cytology
title A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T14%3A51%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Versatile%20Bioreactor%20for%20Dynamic%20Suspension%20Cell%20Culture.%20Application%20to%20the%20Culture%20of%20Cancer%20Cell%20Spheroids&rft.jtitle=PloS%20one&rft.au=Massai,%20Diana&rft.date=2016-05-04&rft.volume=11&rft.issue=5&rft.spage=e0154610&rft.epage=e0154610&rft.pages=e0154610-e0154610&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0154610&rft_dat=%3Cproquest_plos_%3E4044902261%3C/proquest_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c559t-27426a8d12426d850edb30167f320dda4b2407f7343ce5406088e5d404a2b82e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1786792952&rft_id=info:pmid/27144306&rfr_iscdi=true