The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter

• Published in: PloS one 2016-05, Vol.11 (5), p.e0155658-e0155658
• Main Authors: Zalecki, Michal, Sienkiewicz, Waldemar, Franke-Radowiecka, Amelia, Klimczuk, Magdalena, Kaleczyc, Jerzy
• Format: Article
• Language: English
• Subjects: Animals; Biology and Life Sciences; Control; Development and progression; Enteric nervous system; Ethics; Galanin; Galanin - genetics; Galanin - metabolism; Ganglia - metabolism; Ganglia - pathology; Gastric emptying; Gastric motility; Gastric Mucosa - metabolism; Gastric Mucosa - pathology; Gastrointestinal motility; Gene expression; Gene Expression Regulation; Genetic aspects; Higher education; Immunocytochemistry; Immunoreactivity; Inflammation; Innervation; Localization; Medicine and Health Sciences; Motility; mRNA; Myenteric plexus; Myenteric Plexus - metabolism; Nervous system; Neurons; Neuropeptides; Neuroplasticity; Pain; Peptic ulcer; Physiological aspects; Physiology; Pigs; Plasticity (neural); Pyloric Antrum - metabolism; Pyloric Antrum - pathology; Pyloric sphincter; Pylorus - innervation; Pylorus - pathology; Real-Time Polymerase Chain Reaction; Receptors; Receptors, Galanin - genetics; Receptors, Galanin - metabolism; Research and Analysis Methods; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Small intestine; Sphincter; Stomach Ulcer - genetics; Stomach Ulcer - metabolism; Stomach Ulcer - pathology; Sus scrofa; Ulcers; Veterinary medicine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0155658

Gastric antrum ulcerations are common disorders occurring in humans and animals. Such localization of ulcers disturbs the gastric emptying process, which is precisely controlled by the pylorus. Galanin (Gal) and its receptors are commonly accepted to participate in the regulation of inflammatory processes and neuronal plasticity. Their role in the regulation of gastrointestinal motility is also widely described. However, there is lack of data considering antral ulcerations in relation to changes in the expression of Gal and GalR1, GalR2, GalR3 receptors in the pyloric wall tissue and galaninergic intramural innervation of the pylorus. Two groups of pigs were used in the study: healthy gilts and gilts with experimentally induced antral ulcers. By double immunocytochemistry percentages of myenteric and submucosal neurons expressing Gal-immunoreactivity were determined in the pyloric wall tissue and in the population of gastric descending neurons supplying the pyloric sphincter (labelled by retrograde Fast Blue neuronal tracer). The percentage of Gal-immunoreactive neurons increased only in the myenteric plexus of the pyloric wall (from 16.14±2.06% in control to 25.5±2.07% in experimental animals), while no significant differences in other neuronal populations were observed between animals of both groups. Real-Time PCR revealed the increased expression of mRNA encoding Gal and GalR1 receptor in the pyloric wall tissue of the experimental animals, while the expression(s) of GalR2 and GalR3 were not significantly changed. The results obtained suggest the involvement of Gal, GalR1 and galaninergic pyloric myenteric neurons in the response of pyloric wall structures to antral ulcerations.