Characterization of Three Venom Peptides from the Spitting Spider Scytodes thoracica

We present the solution-state NMR structures and preliminary functional characterizations of three venom peptides identified from the spitting spider Scytodes thoracica. Despite little sequence identity to other venom peptides, structural characterization reveals that these peptides contain an inhib...

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Bibliographic Details
Published in:PloS one 2016-05, Vol.11 (5), p.e0156291-e0156291
Main Authors: Ariki, Nathanial K, Muñoz, Lisa E, Armitage, Elizabeth L, Goodstein, Francesca R, George, Kathryn G, Smith, Vanessa L, Vetter, Irina, Herzig, Volker, King, Glenn F, Loening, Nikolaus M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bioinformatics
Biology and Life Sciences
Chemical bonds
Chemical properties
Chemistry
Cystine
Cystine Knot Motifs
Gryllidae - drug effects
Gryllidae - growth & development
Gryllidae - metabolism
Ion channels
Medicine and Health Sciences
Models, Molecular
NMR
Nuclear magnetic resonance
Peptide Fragments - chemistry
Peptide Fragments - pharmacology
Peptides
Physical Sciences
Physiological aspects
Predatory Behavior
Properties
Protein Conformation
Proteins
Receptors
Research and Analysis Methods
Saliva - metabolism
Sequence Homology, Amino Acid
Spider Venoms - chemistry
Spider Venoms - pharmacology
Spiders
Spiders - growth & development
Spiders - metabolism
Structural analysis
Thoracica - drug effects
Toxins
Venom
Venoms
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Description
Summary:We present the solution-state NMR structures and preliminary functional characterizations of three venom peptides identified from the spitting spider Scytodes thoracica. Despite little sequence identity to other venom peptides, structural characterization reveals that these peptides contain an inhibitor cystine knot motif common to many venom peptides. These are the first structures for any peptide or protein from spiders of the Scytodidae family. Many venom peptides target neuronal ion channels or receptors. However, we have not been able to determine the target of these Scytodes peptides so we can only state with certainty the channels and receptors that they do not target.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0156291