Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions

Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL...

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Bibliographic Details
Published in:PloS one 2016-06, Vol.11 (6), p.e0156454-e0156454
Main Authors: Skvortsova, Yulia V, Kondratieva, Sofia A, Zinovyeva, Marina V, Nikolaev, Lev G, Azhikina, Tatyana L, Gainetdinov, Ildar V
Format: Article
Language:English
Subjects:
Argonaute Proteins - genetics
Biology and Life Sciences
Cell cycle
Cell Line, Tumor
Chromatin
Chromatin - metabolism
Computer Simulation
Deoxyribonucleic acid
DNA
DNA binding proteins
Enhancer Elements, Genetic
Enhancers
Epigenetics
Exons
Gene expression
Genetic aspects
Genetic regulation
Genomes
Genomics
Humans
Medical research
Medicine and Health Sciences
Physiological aspects
Poly A - metabolism
Polyadenylation
Promoter Regions, Genetic
Promoters (Genetics)
Proteins
Research and Analysis Methods
RNA, Messenger - metabolism
Testicular cancer
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Summary:Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL2 alternative promoter in exon 7 also functions as an enhancer for gene PHYHIP located 60Kb upstream. This finding of an intragenic enhancer serving as a promoter for a shorter protein isoform implies broader impact on understanding enhancer-promoter networks in regulation of gene expression.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0156454