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Genomic Diversity of Mycobacterium tuberculosis Complex Strains in Cantabria (Spain), a Moderate TB Incidence Setting
Tuberculosis (TB) control strategies are focused mainly on prevention, early diagnosis, compliance to treatment and contact tracing. The objectives of this study were to explore the frequency and risk factors of recent transmission of clinical isolates of Mycobacterium tuberculosis complex (MTBC) in...
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| Published in: | PloS one 2016-06, Vol.11 (6), p.e0157266-e0157266 |
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| container_end_page | e0157266 |
| container_issue | 6 |
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| creator | Pérez Del Molino Bernal, Inmaculada C Lillebaek, Troels Pedersen, Mathias K Martinez-Martinez, Luis Folkvardsen, Dorte B Agüero, Jesús Rasmussen, E Michael |
| description | Tuberculosis (TB) control strategies are focused mainly on prevention, early diagnosis, compliance to treatment and contact tracing. The objectives of this study were to explore the frequency and risk factors of recent transmission of clinical isolates of Mycobacterium tuberculosis complex (MTBC) in Cantabria in Northern Spain from 2012 through 2013 and to analyze their clonal complexity for better understanding of the transmission dynamics in a moderate TB incidence setting.
DNA from 85 out of 87 isolates from bacteriologically confirmed cases of MTBC infection were extracted directly from frozen stocks and genotyped using the mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) method. The MIRU-VNTRplus database tool was used to identify clusters and lineages and to build a neighbor joining (NJ) phylogenetic tree. In addition, data were compared to the SITVIT2 database at the Pasteur Institute of Guadeloupe.
The rate of recent transmission was calculated to 24%. Clustering was associated with being Spanish-born. A high prevalence of isolates of the Euro-American lineage was found. In addition, MIRU-VNTR profiles of the studied isolates corresponded to previously found MIRU-VNTR types in other countries, including Spain, Belgium, Great Britain, USA, Croatia, South Africa and The Netherlands. Six of the strains analyzed represented clonal variants.
Transmission of MTBC is well controlled in Cantabria. The majority of TB patients were born in Spain. The population structure of MTBC in Cantabria has a low diversity of major clonal lineages with the Euro-American lineage predominating. |
| doi_str_mv | 10.1371/journal.pone.0157266 |
| format | article |
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DNA from 85 out of 87 isolates from bacteriologically confirmed cases of MTBC infection were extracted directly from frozen stocks and genotyped using the mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) method. The MIRU-VNTRplus database tool was used to identify clusters and lineages and to build a neighbor joining (NJ) phylogenetic tree. In addition, data were compared to the SITVIT2 database at the Pasteur Institute of Guadeloupe.
The rate of recent transmission was calculated to 24%. Clustering was associated with being Spanish-born. A high prevalence of isolates of the Euro-American lineage was found. In addition, MIRU-VNTR profiles of the studied isolates corresponded to previously found MIRU-VNTR types in other countries, including Spain, Belgium, Great Britain, USA, Croatia, South Africa and The Netherlands. Six of the strains analyzed represented clonal variants.
Transmission of MTBC is well controlled in Cantabria. The majority of TB patients were born in Spain. The population structure of MTBC in Cantabria has a low diversity of major clonal lineages with the Euro-American lineage predominating.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0157266</identifier><identifier>PMID: 27315243</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Biology and Life Sciences ; Biomedical research ; Clinical isolates ; Clustering ; Complexity ; Contact tracing ; Deoxyribonucleic acid ; Disease transmission ; DNA ; Earth Sciences ; Ecology and Environmental Sciences ; Epidemiology ; Female ; Genotype ; Hospitals ; Humans ; Incidence ; Infections ; Laboratories ; Male ; Mathematical analysis ; Medicine and Health Sciences ; Microbiology ; Middle Aged ; Minisatellite Repeats - genetics ; Molecular biology ; Molecular Epidemiology ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - pathogenicity ; People and Places ; Phylogenetics ; Phylogeny ; Population ; Population structure ; Research and Analysis Methods ; Risk analysis ; Risk factors ; Spain ; Strains (organisms) ; Tuberculosis ; Tuberculosis - epidemiology ; Tuberculosis - genetics ; Tuberculosis - microbiology</subject><ispartof>PloS one, 2016-06, Vol.11 (6), p.e0157266-e0157266</ispartof><rights>2016 Pérez del Molino Bernal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016 Pérez del Molino Bernal et al 2016 Pérez del Molino Bernal et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-62bc04758cb276b04e4e8b7d70452bee84a0040abe68cdfff521114d7e426d3b3</citedby><cites>FETCH-LOGICAL-c559t-62bc04758cb276b04e4e8b7d70452bee84a0040abe68cdfff521114d7e426d3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1797683628/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1797683628?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,25734,27905,27906,36993,36994,44571,53772,53774,74875</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27315243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lai, Hsin-Chih</contributor><creatorcontrib>Pérez Del Molino Bernal, Inmaculada C</creatorcontrib><creatorcontrib>Lillebaek, Troels</creatorcontrib><creatorcontrib>Pedersen, Mathias K</creatorcontrib><creatorcontrib>Martinez-Martinez, Luis</creatorcontrib><creatorcontrib>Folkvardsen, Dorte B</creatorcontrib><creatorcontrib>Agüero, Jesús</creatorcontrib><creatorcontrib>Rasmussen, E Michael</creatorcontrib><title>Genomic Diversity of Mycobacterium tuberculosis Complex Strains in Cantabria (Spain), a Moderate TB Incidence Setting</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Tuberculosis (TB) control strategies are focused mainly on prevention, early diagnosis, compliance to treatment and contact tracing. The objectives of this study were to explore the frequency and risk factors of recent transmission of clinical isolates of Mycobacterium tuberculosis complex (MTBC) in Cantabria in Northern Spain from 2012 through 2013 and to analyze their clonal complexity for better understanding of the transmission dynamics in a moderate TB incidence setting.
DNA from 85 out of 87 isolates from bacteriologically confirmed cases of MTBC infection were extracted directly from frozen stocks and genotyped using the mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) method. The MIRU-VNTRplus database tool was used to identify clusters and lineages and to build a neighbor joining (NJ) phylogenetic tree. In addition, data were compared to the SITVIT2 database at the Pasteur Institute of Guadeloupe.
The rate of recent transmission was calculated to 24%. Clustering was associated with being Spanish-born. A high prevalence of isolates of the Euro-American lineage was found. In addition, MIRU-VNTR profiles of the studied isolates corresponded to previously found MIRU-VNTR types in other countries, including Spain, Belgium, Great Britain, USA, Croatia, South Africa and The Netherlands. Six of the strains analyzed represented clonal variants.
Transmission of MTBC is well controlled in Cantabria. The majority of TB patients were born in Spain. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez Del Molino Bernal, Inmaculada C</au><au>Lillebaek, Troels</au><au>Pedersen, Mathias K</au><au>Martinez-Martinez, Luis</au><au>Folkvardsen, Dorte B</au><au>Agüero, Jesús</au><au>Rasmussen, E Michael</au><au>Lai, Hsin-Chih</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic Diversity of Mycobacterium tuberculosis Complex Strains in Cantabria (Spain), a Moderate TB Incidence Setting</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>11</volume><issue>6</issue><spage>e0157266</spage><epage>e0157266</epage><pages>e0157266-e0157266</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Tuberculosis (TB) control strategies are focused mainly on prevention, early diagnosis, compliance to treatment and contact tracing. The objectives of this study were to explore the frequency and risk factors of recent transmission of clinical isolates of Mycobacterium tuberculosis complex (MTBC) in Cantabria in Northern Spain from 2012 through 2013 and to analyze their clonal complexity for better understanding of the transmission dynamics in a moderate TB incidence setting.
DNA from 85 out of 87 isolates from bacteriologically confirmed cases of MTBC infection were extracted directly from frozen stocks and genotyped using the mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) method. The MIRU-VNTRplus database tool was used to identify clusters and lineages and to build a neighbor joining (NJ) phylogenetic tree. In addition, data were compared to the SITVIT2 database at the Pasteur Institute of Guadeloupe.
The rate of recent transmission was calculated to 24%. Clustering was associated with being Spanish-born. A high prevalence of isolates of the Euro-American lineage was found. In addition, MIRU-VNTR profiles of the studied isolates corresponded to previously found MIRU-VNTR types in other countries, including Spain, Belgium, Great Britain, USA, Croatia, South Africa and The Netherlands. Six of the strains analyzed represented clonal variants.
Transmission of MTBC is well controlled in Cantabria. The majority of TB patients were born in Spain. The population structure of MTBC in Cantabria has a low diversity of major clonal lineages with the Euro-American lineage predominating.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27315243</pmid><doi>10.1371/journal.pone.0157266</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2016-06, Vol.11 (6), p.e0157266-e0157266 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1797683628 |
| source | Publicly Available Content Database; PubMed Central |
| subjects | Adult Biology and Life Sciences Biomedical research Clinical isolates Clustering Complexity Contact tracing Deoxyribonucleic acid Disease transmission DNA Earth Sciences Ecology and Environmental Sciences Epidemiology Female Genotype Hospitals Humans Incidence Infections Laboratories Male Mathematical analysis Medicine and Health Sciences Microbiology Middle Aged Minisatellite Repeats - genetics Molecular biology Molecular Epidemiology Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - pathogenicity People and Places Phylogenetics Phylogeny Population Population structure Research and Analysis Methods Risk analysis Risk factors Spain Strains (organisms) Tuberculosis Tuberculosis - epidemiology Tuberculosis - genetics Tuberculosis - microbiology |
| title | Genomic Diversity of Mycobacterium tuberculosis Complex Strains in Cantabria (Spain), a Moderate TB Incidence Setting |
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