Field Performance of Bt Eggplants (Solanum melongena L.) in the Philippines: Cry1Ac Expression and Control of the Eggplant Fruit and Shoot Borer (Leucinodes orbonalis Guenée)

• Published in: PloS one 2016-06, Vol.11 (6), p.e0157498
• Main Authors: Hautea, Desiree M, Taylo, Lourdes D, Masanga, Anna Pauleen L, Sison, Maria Luz J, Narciso, Josefina O, Quilloy, Reynaldo B, Hautea, Randy A, Shotkoski, Frank A, Shelton, Anthony M
• Format: Article
• Language: English
• Subjects: Agriculture; Animals; Asia; Bacillus thuringiensis; Bacillus thuringiensis - genetics; Bacillus thuringiensis Toxins; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Biology and Life Sciences; Borers; Butterflies & moths; Comparators; Corn; Cotton; Crops; Crops, Agricultural - genetics; Crops, Agricultural - growth & development; Cry1Ac toxin; Eggs; Endotoxins - biosynthesis; Endotoxins - genetics; Engineering and Technology; Farms; Fruit - genetics; Fruit - growth & development; Fruit - microbiology; Fruit - parasitology; Fruits; Gene Expression Regulation, Plant; Hemolysin Proteins - biosynthesis; Hemolysin Proteins - genetics; Infestation; Insect pests; Insecticides; Insects; Larva - pathogenicity; Larvae; Leaves; Leucinodes orbonalis; Moths; Moths - pathogenicity; Offspring; Pest control; Pest Control, Biological; Pests; Philippines; Plant Leaves - genetics; Plant Leaves - growth & development; Plant Leaves - microbiology; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant resistance; Plant Shoots - genetics; Plant Shoots - growth & development; Plant Shoots - microbiology; Proteins; Research and Analysis Methods; Shoots; Solanum melongena; Solanum melongena - genetics; Solanum melongena - growth & development; Solanum melongena - microbiology; Solanum melongena - parasitology; Transformation; Vegetables; Zea mays
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0157498

Plants expressing Cry proteins from the bacterium, Bacillus thuringiensis (Bt), have become a major tactic for controlling insect pests in maize and cotton globally. However, there are few Bt vegetable crops. Eggplant (Solanum melongena) is a popular vegetable grown throughout Asia that is heavily treated with insecticides to control the eggplant fruit and shoot borer, Leucinodes orbonalis (EFSB). Herein we provide the first publicly available data on field performance in Asia of eggplant engineered to produce the Cry1Ac protein. Replicated field trials with five Bt eggplant open-pollinated (OP) lines from transformation event EE-1 and their non-Bt comparators were conducted over three cropping seasons in the Philippines from 2010-2012. Field trials documented levels of Cry1Ac protein expressed in plants and evaluated their efficacy against the primary target pest, EFSB. Cry1Ac concentrations ranged from 0.75-24.7 ppm dry weight with the highest in the terminal leaves (or shoots) and the lowest in the roots. Cry1Ac levels significantly increased from the vegetative to the reproductive stage. Bt eggplant lines demonstrated excellent control of EFSB. Pairwise analysis of means detected highly significant differences between Bt eggplant lines and their non-Bt comparators for all field efficacy parameters tested. Bt eggplant lines demonstrated high levels of control of EFSB shoot damage (98.6-100%) and fruit damage (98.1-99.7%) and reduced EFSB larval infestation (95.8-99.3%) under the most severe pest pressure during trial 2. Moths that emerged from larvae collected from Bt plants in the field and reared in their Bt eggplant hosts did not produce viable eggs or offspring. These results demonstrate that Bt eggplant lines containing Cry1Ac event EE-1 provide outstanding control of EFSB and can dramatically reduce the need for conventional insecticides.