Characterization of Mammalian ADAM2 and Its Absence from Human Sperm

The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other...

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Published in:PloS one 2016-06, Vol.11 (6), p.e0158321-e0158321
Main Authors: Choi, Heejin, Jin, Sora, Kwon, Jun Tae, Kim, Jihye, Jeong, Juri, Kim, Jaehwan, Jeon, Suyeon, Park, Zee Yong, Jung, Kang-Jin, Park, Kwangsung, Cho, Chunghee
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Analysis
Animals
Antibodies
Biology and Life Sciences
Chemical properties
Chromatography, Liquid
Epidermal growth factor
Failure analysis
Fertilins - chemistry
Fertilins - genetics
Fertilins - metabolism
Germ cells
Humans
Immunoglobulins
Life sciences
Macaca fascicularis
Male
Mammals
Mammals - metabolism
Medicine and Health Sciences
Metalloproteinase
Mice
Monkeys
Physiological aspects
Protein Interaction Domains and Motifs
Proteins
Proteomics
Reproduction
Reproductive system
Research and Analysis Methods
Sperm
Spermatozoa - metabolism
Tandem Mass Spectrometry
Testis - metabolism
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cited_by cdi_FETCH-LOGICAL-c725t-d700e019ceb32a471d4cd804d36e758fea1ad68c22772af9cf1899d300a811863
cites cdi_FETCH-LOGICAL-c725t-d700e019ceb32a471d4cd804d36e758fea1ad68c22772af9cf1899d300a811863
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container_issue 6
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container_title PloS one
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creator Choi, Heejin
Jin, Sora
Kwon, Jun Tae
Kim, Jihye
Jeong, Juri
Kim, Jaehwan
Jeon, Suyeon
Park, Zee Yong
Jung, Kang-Jin
Park, Kwangsung
Cho, Chunghee
description The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other ADAMs form complexes that contribute to sperm-sperm adhesion, sperm-egg interactions, and the migration of sperm in the female reproductive tract. Here, we generated specific antibodies against mouse and human ADAM2, and investigated various features of ADAM2 in mice, monkeys and humans. We found that the cytoplasmic domain of ADAM2 might enable the differential association of this protein with other ADAMs in mice. Western blot analysis with the anti-human ADAM2 antibodies showed that ADAM2 is present in the testis and sperm of monkeys. Monkey ADAM2 was found to associate with chaperone proteins in testis. In humans, we identified ADAM2 as a 100-kDa protein in the testis, but failed to detect it in sperm. This is surprising given the results in mice and monkeys, but it is consistent with the failure of ADAM2 identification in the previous proteomic analyses of human sperm. These findings suggest that the reproductive functions of ADAM2 differ between humans and mice. Our protein analysis showed the presence of potential ADAM2 complexes involving yet-unknown proteins in human testis. Taken together, our results provide new information regarding the characteristics of ADAM2 in mammalian species, including humans.
doi_str_mv 10.1371/journal.pone.0158321
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This is surprising given the results in mice and monkeys, but it is consistent with the failure of ADAM2 identification in the previous proteomic analyses of human sperm. These findings suggest that the reproductive functions of ADAM2 differ between humans and mice. Our protein analysis showed the presence of potential ADAM2 complexes involving yet-unknown proteins in human testis. Taken together, our results provide new information regarding the characteristics of ADAM2 in mammalian species, including humans.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>27341348</pmid><doi>10.1371/journal.pone.0158321</doi><tpages>e0158321</tpages><oa>free_for_read</oa></addata></record>
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subjects Amino Acid Sequence
Amino acids
Analysis
Animals
Antibodies
Biology and Life Sciences
Chemical properties
Chromatography, Liquid
Epidermal growth factor
Failure analysis
Fertilins - chemistry
Fertilins - genetics
Fertilins - metabolism
Germ cells
Humans
Immunoglobulins
Life sciences
Macaca fascicularis
Male
Mammals
Mammals - metabolism
Medicine and Health Sciences
Metalloproteinase
Mice
Monkeys
Physiological aspects
Protein Interaction Domains and Motifs
Proteins
Proteomics
Reproduction
Reproductive system
Research and Analysis Methods
Sperm
Spermatozoa - metabolism
Tandem Mass Spectrometry
Testis - metabolism
title Characterization of Mammalian ADAM2 and Its Absence from Human Sperm
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