Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study

Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calcium-regulatable protein phosphata...

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Published in:PloS one 2016-06, Vol.11 (6), p.e0158424-e0158424
Main Authors: Petrezsélyová, Silvia, López-Malo, María, Canadell, David, Roque, Alicia, Serra-Cardona, Albert, Marqués, M Carmen, Vilaprinyó, Ester, Alves, Rui, Yenush, Lynne, Ariño, Joaquín
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus - genetics
Baking yeast
Binding sites
Binding Sites - genetics
Biology and life sciences
Calcineurin
Calcineurin - metabolism
Calcineurin - physiology
Calcium
Cell Nucleus - genetics
Cell Nucleus - metabolism
DNA-Binding Proteins - metabolism
DNA-Binding Proteins - physiology
Engineering and Technology
Gene expression
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Hydrogen-Ion Concentration
Kinases
Kinetics
Mathematical models
Na+/K+-exchanging ATPase
Organisms, Genetically Modified
pH effects
Phosphatase
Physical Sciences
Promoter Regions, Genetic
Protein phosphatase
Protein Transport
Proteins
Quantitative research
Regulatory Elements, Transcriptional
Research and Analysis Methods
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Saccharomyces cerevisiae Proteins - physiology
Signaling
Sodium-Potassium-Exchanging ATPase - genetics
Sodium-Potassium-Exchanging ATPase - metabolism
Stress
Stress, Physiological - genetics
Stresses
Transcription factors
Transcription Factors - metabolism
Transcription Factors - physiology
Yeast
Yeasts
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cited_by cdi_FETCH-LOGICAL-c559t-603020fa9dae809f5e69dc183a19175e337f0f05235d03e154b1629258bf8ce93
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creator Petrezsélyová, Silvia
López-Malo, María
Canadell, David
Roque, Alicia
Serra-Cardona, Albert
Marqués, M Carmen
Vilaprinyó, Ester
Alves, Rui
Yenush, Lynne
Ariño, Joaquín
description Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calcium-regulatable protein phosphatase calcineurin and its downstream transcription factor Crz1. We present here a quantitative study of the expression of Ena1 in response to alkalinization of the environment and we analyze the contribution of Crz1 to this response. Experimental data and mathematical models substantiate the existence of two stress-responsive Crz1-binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early response of the ENA1 promoter is about 60%. The models suggest the existence of a second input with similar kinetics, which would be likely mediated by high pH-induced activation of the Snf1 kinase.
doi_str_mv 10.1371/journal.pone.0158424
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subjects Active Transport, Cell Nucleus - genetics
Baking yeast
Binding sites
Binding Sites - genetics
Biology and life sciences
Calcineurin
Calcineurin - metabolism
Calcineurin - physiology
Calcium
Cell Nucleus - genetics
Cell Nucleus - metabolism
DNA-Binding Proteins - metabolism
DNA-Binding Proteins - physiology
Engineering and Technology
Gene expression
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Hydrogen-Ion Concentration
Kinases
Kinetics
Mathematical models
Na+/K+-exchanging ATPase
Organisms, Genetically Modified
pH effects
Phosphatase
Physical Sciences
Promoter Regions, Genetic
Protein phosphatase
Protein Transport
Proteins
Quantitative research
Regulatory Elements, Transcriptional
Research and Analysis Methods
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Saccharomyces cerevisiae Proteins - physiology
Signaling
Sodium-Potassium-Exchanging ATPase - genetics
Sodium-Potassium-Exchanging ATPase - metabolism
Stress
Stress, Physiological - genetics
Stresses
Transcription factors
Transcription Factors - metabolism
Transcription Factors - physiology
Yeast
Yeasts
title Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study
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