Atrial Natriuretic Peptide Stimulates Dopamine Tubular Transport by Organic Cation Transporters: A Novel Mechanism to Enhance Renal Sodium Excretion

• Published in: PloS one 2016-07, Vol.11 (7), p.e0157487-e0157487
• Main Authors: Kouyoumdzian, Nicolás M, Rukavina Mikusic, Natalia L, Kravetz, María C, Lee, Brenda M, Carranza, Andrea, Del Mauro, Julieta S, Pandolfo, Marcela, Gironacci, Mariela M, Gorzalczany, Susana, Toblli, Jorge E, Fernández, Belisario E, Choi, Marcelo R
• Format: Article
• Language: English
• Subjects: Animal tissues; Animals; Atrial Natriuretic Factor - metabolism; Atrial natriuretic peptide; Biological Transport; Biology and Life Sciences; Cation Transport Proteins - metabolism; Cations; Cell Membrane - metabolism; Chromatography, High Pressure Liquid; Diuresis; Diuresis - drug effects; Diuretics; Dopamine; Dopamine - metabolism; Dopamine - urine; Dopamine D1 receptors; Dopamine transporter; Excretion; Genetic aspects; High-performance liquid chromatography; Kidney - metabolism; Kidney Tubules - metabolism; Kinases; Liquid chromatography; Male; Medicine and Health Sciences; Na+/K+-exchanging ATPase; Natriuresis - drug effects; Natriuretic peptides; Pargyline; Physical Sciences; Physiological aspects; Protein kinase; Protein kinase G; Random Allocation; Rats; Rats, Sprague-Dawley; Renal cortex; Renal function; Research and Analysis Methods; Rodents; Saline solutions; Signal transduction; Signaling; Sodium; Sodium - metabolism; Sodium-Potassium-Exchanging ATPase - metabolism; Tolcapone; Transport
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0157487

The aim of this study was to demonstrate the effects of atrial natriuretic peptide (ANP) on organic cation transporters (OCTs) expression and activity, and its consequences on dopamine urinary levels, Na+, K+-ATPase activity and renal function. Male Sprague Dawley rats were infused with isotonic saline solution during 120 minutes and randomized in nine different groups: control, pargyline plus tolcapone (P+T), ANP, dopamine (DA), D-22, DA+D-22, ANP+D-22, ANP+DA and ANP+DA+D-22. Renal functional parameters were determined and urinary dopamine concentration was quantified by HPLC. Expression of OCTs and D1-receptor in membrane preparations from renal cortex tissues were determined by western blot and Na+, K+-ATPase activity was determined using in vitro enzyme assay. 3H-DA renal uptake was determined in vitro. Compared to P+T group, ANP and dopamine infusion increased diuresis, urinary sodium and dopamine excretion significantly. These effects were more pronounced in ANP+DA group and reversed by OCTs blockade by D-22, demonstrating that OCTs are implied in ANP stimulated-DA uptake and transport in renal tissues. The activity of Na+, K+-ATPase exhibited a similar fashion when it was measured in the same experimental groups. Although OCTs and D1-receptor protein expression were not modified by ANP, OCTs-dependent-dopamine tubular uptake was increased by ANP through activation of NPR-A receptor and protein kinase G as signaling pathway. This effect was reflected by an increase in urinary dopamine excretion, natriuresis, diuresis and decreased Na+, K+-ATPase activity. OCTs represent a novel target that links the activity of ANP and dopamine together in a common mechanism to enhance their natriuretic and diuretic effects.